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Wednesday, June 11, 2008

Biochemical Resource --(2)

Alzheimer's Disease - Vaccine Prevents Alzheimer's In Mice (applesforhealth)


Basic Biological Structure Revealed (applesforhealth)


Brain - Compound Fights Prion Brain Disease (applesforhealth)


Brain - Probing The Mechanics Of Memory (applesforhealth)


Brain Region Mapped (applesforhealth)


Cancer - Repairing DNA After UV Damage (applesforhealth)


Cancer - Smart Bombs May Blast Cancer Cells (applesforhealth)


Cancer - Unmasking Genetic Causes Of Cancer (applesforhealth)


Cell Transplant Fixes Damaged Brain (applesforhealth)


Biomedia http://ebiomedia.com


The Biology Project: Biochemistry


Chemistry Tutorial


BIOCHEMISTRY TUTORIAL


General, Organic and Biochemistry


www.kumc.edu/research/medicine/biochemistry/bioc800/start.html


AW: Tutorial


Tutorials


biochemistry tutorial, biochemistry online tutorial


Beginning Molecular Biology Laboratory Manual


Biochemistry (Virtual Textbook to accompany Matthews and van Holde edition)


Bioinformatics Courses Around the World


Biol. 2421 Lab Manual - Table of Contents


Cell Biology Laboratory Manual


DNA Isolation Procedures


DNA Purification Protocols and Applications Guide


Electronic Laboratory Manual


ESG Biology Hypertextbook


Kimball's Biology Pages


Medical Biochemistry Subject List


Molecular Biology of the Cell (4th ed)


Molecular Biology Techniques Manual


Molecular Biology Web Book


Molecular Cell Biology (6th ed)


NetBiochem


Online Biology Book


Online Microbiology Lab Manual


Plant Physiology online: A Companion to Plant Physiology, (4th ed)


Principles of Protein Structure Using the Internet


RABINOVITCH PROTOCOL MANUAL


The Science Creative Quarterly, Cell Biology text book page


Western Blotting Handbook and Troubleshooting Guid

Biochemical Resource--(1)


Amino Acid Properties


Beginner's Guide to Molecular Biology


Biochemistry: Course Description


Biochemistry - Web Edition


Biochemistry and Cell Biology


Biochemistry Course Materials


Biochemistry of Neurotransmitters


Biochemistry: Study Guide


Biology Online


The Biology Project: Biochemistry


Biophysical Chemistry


Center for Molecular Modeling


Computational Biochemistry Server at ETHZ


DNA Graphics


European Journal of Biochemistry


Glossary of Biochemistry and Molecular Biology


Graphics Gallery


Images of Proteins/DNA


Kansas University Medical Center Resources for Biochemistry


Life Science Directory


Metabolic Pathways of Biochemistry


Molecular Library


Molecular Models for Biochemistry


NDB Atlas of Nucleic Acid Containing Structures


Cells


Lessons Plans in Science


Biochemistry 1 (BioChemNet - Schmidel)


Biochemistry 2 (BioChemNet - Schmidel)


Biotechnology (BioChemNet - Schmidel)


Biotechnology Dictionary (BioTech Resources)


Cells - Dictionary of Cell and Molecular Biology (Lackey and Dow) 3-00


Genomes Research - Resources (US Department of Energy and the Human Genome Project)


Human Genome Map - Chromosome 21 and Base Pairs (BBC News)


Human Genome Map - Status (Nature - Macmillan Publishers)


Biology Clip Art - Free (BioChemNet - Schmidel)


DNA Sequencing (Howard Hughes Medical Institute)


DNA, Genes, and Heredity (DNA Learning Center)


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Useful tools and softwares for biochemistry

A comprehensive database of molecular structures
http://www.biomednet.com/db/msd

A biocomputing hypertext coursebook
http://merlin.mbcr.bcm.tmc.edu:8001/bcd/Curric/welcome.html

A molecular graphics art show
http://www.scripps.edu/pub/goodsell/mgs_art/mgs_art.html

A more recent molecular graphics art show
http://www.scripps.edu/pub/goodsell/mgs_art/mgs_art2/index.html

The Protein Data Bank through Microsoft Windows
http://www.sdsc.edu/pb/wpdb/wpdb.htm

Software for biological data representation
http://www.sdsc.edu/pb/Software.html

Home page for a molecular graphics laboratory
http://www.scripps.edu/pub/olson-web/

Hierarchical classification of proteins
http://www.protomap.cs.huji.ac.il/

Biocomputing and molecular modeling
http://www-bio.unizh.ch/

Computational molecular biology at NIH
http://webasaurus.dcrt.nih.gov/molbio/

Online resources for molecular biology
http://webasaurus.dcrt.nih.gov/molbio/servers.html

A collection of biomolecular research tools
http://www.public.iastate.edu/~pedro/research_tools.html

Molecular models for biochemistry
http://info.bio.cmu.edu/Courses/BiochemMols/BCMolecules.html

Image library of biological macromolecules
http://www.imb-jena.de/IMAGE.html

The "Ribbons" software home page
http://www.cmc.uab.edu/ribbons/

PovChem program for molecular graphics
http://grserv.med.jhmi.edu/~paul/Manual.html

The MolMol program for molecular visualization
http://www.mol.biol.ethz.ch/wuthrich/software/molmol/

Molecular biology database compendium
http://www.tulane.edu/~dmsander/biotechniquessites.html

Some biochemistry molecular models
http://moby.ucdavis.edu/HRM/Biochemistry/molecules.htm

A molecular library
http://c4.cabrillo.cc.ca.us/projects/library/

Animated and still biochemistry graphics
http://www-medlib.med.utah.edu/NetBiochem/graphlis.htm

Online macromolecular museum
http://www.clunet.edu/BioDev/omm/gallery.htm

An art gallery of molecular models
http://www2.kenyon.edu/depts/biology/eward/gallery/galleryintro.html

Entrez: Data sets for biochemistry and molecular biology.
http://www3.ncbi.nlm.nih.gov/Entrez/index.html

MolGraph: Molecular modeling program.
http://chemb16.leidenuniv.nl/~armand/

Swiss PDB viewer
http://expasy.expasy.ch/spdbv/

NIH Center for Molecular Modeling
http://cmm.info.nih.gov/modeling/

Virtual reality and biochemistry
http://www.tc.cornell.edu:80/~richard/

Biomolecular Visualization Center
http://amanda.uams.edu/index_text.html

VRML file creator for chemical structures
http://www2.ccc.uni-erlangen.de/services/vrmlcreator/

Rendering techniques for molecular models
http://scsg9.unige.ch/fln/eng/toc.html

The Journal of Molecular Modeling
http://link.springer.de/link/service/journals/00894/index.htm

Using NMR for biomolecular structures
http://www.ocms.ox.ac.uk/idc/structures/

Biomolecular structure and modeling in the United Kingdom
http://www.biochem.ucl.ac.uk/bsm/

Molecular Models Online
http://www.webmolecules.com/index.shtml

Molecular visualization tools and sites
http://www.indiana.edu:80/~cheminfo/mvts.html

RCSB: The home site for all PDB files.
http://www.rcsb.org/pdb/

Monday, June 9, 2008

Biochemistry Protocols Online (part 1)

General Lab Techniques

Lab security and basic techniques

Advanced lab skills-(1)

Advanced lab skills-(2)

Molecular Biology

DNA isolation & related protocols

DNA Purification (glass milk vs electroelution)

DNA and RNA sequencing

Nucleic acid methods (1)

Nucleic acid methods (2)

Isolation of DNA,RNA, and Protein simultaneously.

DNA mutation detection by SSCP

Mouse genotyping by PCR

PCR,RT-PCR,Real time PCR etc.

Southern blot hybridization

Loss of Heterozygosity (LOH)

Gene knockout protocol

RNA Isolation and Purification

Preparation of DNA and RNA probes

Northern blot hybridization

SiRNA gene knockout

Western blot hybridization

Molecular cloning

Conditional gene transfection(Tet on/off)

Protein sequencing

Protein labeling techniques

Subcellular fractionations

EMSA

Methylation interference

Southwestern blotting

Filter binding protocols

Footpring protocols

DNA (RNA) protein interactions

Plasmid and its usefulness

DNA library construction

Microarray protocols.

Protein chips

Methods for detecting protein phosphorylation

Protein methods

Molecular separation

Gene therapy for cancer

X-ray crystallography

Metabolism and chromatographies

GC/MS Background

Glucose metabolism and its related protocols

PAS staining

Polysaccharide sequencing

Deoxyribose procedure

Ribose metabolism analysis

Lactate cleanup and derivative

Amino acids

Fatty acids

Lipid protocols

Cholesterol

Bile acids

Urea procedure

Choline Incorporation Assay

Isotope Ratio Mass Spectrometer (IRMS)

Liquid chromatography / mass spectrometer (LC/MS)

Proteomics

NMR protocols and tutorials

HPLC protocols

TLC

Molecular labeling

Biochemistry Protocols Online (part 2)

Immunology/immunohistochemistry

Preparing silanized (+plus) slides

Microscopy Techniques

Electron microscopy

HE staining

Nucleic acid stain

Special cell & cell fraction stains

Antibody purification

Antibody storage and handling

Conjugation of monoclonal antibodies

Antigen retrieval

Elisa

FRQs for histochemistry

Immunoperoxidase staining techniques

Immunofluorohistochemistry

Confocal microscope technique

Laser Capture Microdissection

Immunoprecipitation

ChIP assay

Hybridization in situ

Histotechnology--technical methods

Flow cytometry (FCM)

Kinase assay

Cellular Biology

General cell culture protocols

Chromosome karyotype

Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)

Cell cycle assay

Migration assay

Stem cell & related protocols

Apoptosis and related protocols

Soft Agar Assay for Colony Formation

Aorta ring assay

GFP transfection

Gene transfection

Transformation protocols

Blood cell fractionation (white blood cell isolation)

Endothelial isolation and culture protocols

Isolation and culture human brain microvessel endothelial cells

Animal Experiments

Blood sampling from animals

Basic skills for animal experiments

Cancer xenograft animal models

Transgenic animal procedures

Transgenic cancer models

Animal search

Animal models for depression-like and anxiety-like behavior

Genetics

Epigenetics protocols

SNPs

Genetic analysis

Mutagenesis

Model Organisms

C. Elegans

Super Biochemistry--unit 1-50

Biochemistry (Chapters and Vocabulary)

DNA Replication, Recombination, and Repair

1: Okazaki Fragments.

2: Priming.

3: Origin of Replication in E. coli.

4: Double-Stranded DNA Can Wrap Around Itself to Form Supercoiled Structures

5: Topoisomerase II Mechanism.

6: Structure of Topoisomerase II.

7: Topoisomerase I Mechanism.

8: Structure of a Topoisomerase.

9: Topoisomers.

10: Linking Number.

11: DNA Polymerases Require a Template and a Primer

12: Conserved Residues among Helicases.

13: Helicase Mechanism.

14: Helicase Structure.

15: Proofreading.

16: Shape Selectivity.

17: Minor-Groove Interactions.

18: DNA Polymerase Mechanism.

19: DNA Polymerase Structure.

20: DNA Can Assume a Variety of Structural Forms

21: Comparison of A-, B-, and Z-DNA

22: Z-DNA.

23: Propeller Twist.

24: Major and Minor Grooves in B-Form DNA.

25: Major- and Minor-Groove Sides.

26: Steric Clash.

27: Sugar Puckers.

28: B-Form and A-Form DNA.

29: Faithful copying is essential to the storage of genetic information.

30: DNA Replication At Low Resolution.

31: Consequences of Strand Separation.

32: DNA Replication.

33: DNA Replication, Recombination, and Repair

34: Problems

35: Summary

36: Mutations Involve Changes in the Base Sequence of DNA

37: Ames Test.

38: Triplet Repeat Expansion.

39: Mismatch Repair.

40: Uracil Repair.

41: Excision Repair.

42: Structure of DNA-Repair Enzyme.

43: Repair Pathways.

44: Cross-Linked Dimer of Two Thymine Bases.

45: Aflatoxin Reaction.

46: Acridines.

47: Chemical Mutagenesis.

48: Base Pair with 5-Bromouracil.

49: Base Pair with Mutagenic Tautomer.

50: Double-Stranded DNA Molecules with Similar Sequences Sometimes Recombine