Biochemistry Protocols Online (part 2)

Immunology/immunohistochemistry

Preparing silanized (+plus) slides

Microscopy Techniques

Electron microscopy

HE staining

Nucleic acid stain

Special cell & cell fraction stains

Antibody purification

Antibody storage and handling

Conjugation of monoclonal antibodies

Antigen retrieval

Elisa

FRQs for histochemistry

Immunoperoxidase staining techniques

Immunofluorohistochemistry

Confocal microscope technique

Laser Capture Microdissection

Immunoprecipitation

ChIP assay

Hybridization in situ

Histotechnology--technical methods

Flow cytometry (FCM)

Kinase assay

Cellular Biology

General cell culture protocols

Chromosome karyotype

Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)

Cell cycle assay

Migration assay

Stem cell & related protocols

Apoptosis and related protocols

Soft Agar Assay for Colony Formation

Aorta ring assay

GFP transfection

Gene transfection

Transformation protocols

Blood cell fractionation (white blood cell isolation)

Endothelial isolation and culture protocols

Isolation and culture human brain microvessel endothelial cells

Animal Experiments

Blood sampling from animals

Basic skills for animal experiments

Cancer xenograft animal models

Transgenic animal procedures

Transgenic cancer models

Animal search

Animal models for depression-like and anxiety-like behavior

Genetics

Epigenetics protocols

SNPs

Genetic analysis

Mutagenesis

Model Organisms

C. Elegans