1.
Dissolve 100ug DNA in 200ul distilled water.
2.
Add MgCL2 and sodium acetate to the final concentration of 15mM and 10mM , respectively.
3.
Incubate with 2 units DNAse I at room temperature for 15 minutes.
4.
Add ammonium bicarbonate to the final concentration of 100mM , and then incubate with
2mU
phosphodiesterase at 37oC in water bath for 2 hours.
5.
Add 1 unit of alkaline phosphatase and incubated at 37oC water bath for another
1 hour.
6.
Air dry the solution with degraded DNA. Derivalize produced deoxyribose to its
aldonitrile
acetate
form as usual for analysis on GC/MS spectrometer.
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