Biochemistry Protocols Online (part 1)

General Lab Techniques

Lab security and basic techniques

Advanced lab skills-(1)

Advanced lab skills-(2)

Molecular Biology

DNA isolation & related protocols

DNA Purification (glass milk vs electroelution)

DNA and RNA sequencing

Nucleic acid methods (1)

Nucleic acid methods (2)

Isolation of DNA,RNA, and Protein simultaneously.

DNA mutation detection by SSCP

Mouse genotyping by PCR

PCR,RT-PCR,Real time PCR etc.

Southern blot hybridization

Loss of Heterozygosity (LOH)

Gene knockout protocol

RNA Isolation and Purification

Preparation of DNA and RNA probes

Northern blot hybridization

SiRNA gene knockout

Western blot hybridization

Molecular cloning

Conditional gene transfection(Tet on/off)

Protein sequencing

Protein labeling techniques

Subcellular fractionations

EMSA

Methylation interference

Southwestern blotting

Filter binding protocols

Footpring protocols

DNA (RNA) protein interactions

Plasmid and its usefulness

DNA library construction

Microarray protocols.

Protein chips

Methods for detecting protein phosphorylation

Protein methods

Molecular separation

Gene therapy for cancer

X-ray crystallography

Metabolism and chromatographies

GC/MS Background

Glucose metabolism and its related protocols

PAS staining

Polysaccharide sequencing

Deoxyribose procedure

Ribose metabolism analysis

Lactate cleanup and derivative

Amino acids

Fatty acids

Lipid protocols

Cholesterol

Bile acids

Urea procedure

Choline Incorporation Assay

Isotope Ratio Mass Spectrometer (IRMS)

Liquid chromatography / mass spectrometer (LC/MS)

Proteomics

NMR protocols and tutorials

HPLC protocols

TLC

Molecular labeling