Web Source of Biochemical Protocols

Web links of bioprotocols (1)

Web links of bioprotocols (2)

Protocols for Protein
1 Enzyme Assay（43-47）
2 Enzyme Assay（36-42）
3 Enzyme Assay（22-28）
4 Protocols for In Vitro Translation
5 Dot Blot Protocols
6 Selected Protocols of Protein Purification
7 Western Blotting Protocols (1)
Protocols for Cells
1 FACS for Bone marrow macrophages
2 Bone Marrow Macrophages
3 Metabolic Labeling of Cells with 35S
4 Protocols from the science advisory board
5 Introduction to Animal Cell Culture
6 Cell Transfection Protocols
7 Laser Capture Microdissection Protocols
8 Stem Cell Research Techniques and Protocols
9 XTT Cell Viability Assay Protocol
10 Apoptosis Assay Protocols
Protocols for Genetics
1 Genetic Analyses for DNA Protein Interactions
2 Comet Assay
3 Epigenetics Protocols
Protocols for Immunology and IHC
1 Grocott's Methenamine Silver (GMS) stain and troubleshooting
2 Elegans Immunohistochemistry
3 Cytokine Assay Protocols
4 Fluorescence in situ hybridization (FISH)
5 Radioimmunoassay--RIA Protocols
6 Competitive ELISA Protocols
7 Immunohistochemistry Stain for Frozen tissue
Protocols for Chromatography
1 Polysaccharide sequencing
2 Application Guides and Protocols (Nest Group)
3 HPLC Troubleshooting
4 Introduction to HPLC
Protocols for Model Organisms
1 Yeast Protocols
Protocols for Animal Experiment
1 Murine Models of Human Breast Cancer (B)
2 Chorioallantoic Membrane Vascular Assay
3 Guidelines for Techniques with Rodents (Duke University)
4 Tail Vein Injection Techniques
5 Animal Search Engines
Protocols for Basic Techniques
1 H & E Stain Troubleshooting
2 H and E Stain Protocols
3 Electrophoresis Protocols (11-26)
4 Lab. Safety in Life Science

Biorotocols for Biochemistry

Protocols for DNA
1 Methylene Blue DNA staining protocol
2 Plasmid Protocols--(31-37)
3 Plasmid Protocols--(21-30)
4 Plasmid Protocol--(1-10)
5 Microsatellites Protocols
6 In-Situ hybridization to Embryonic Tissue Section
7 DNA ligation Protocol
8 Colony Hybridization Protocols
9 Colony Hybridization
10 DNA Isolation, Purification and Troubleshooting
11 Deoxyribose Isolation from DNA Degrasion
12 Mitochondrial DNA Isolation
13 Genomic DNA Isolation from Specific Samples
14 Genome DNA Extraction
Protocols for RNA
1 IN VITRO TRANSCRIPTION OF RNA
2 SINGLE CELL mRNA AMPLIFICATION (REVERSE NORTHERN ANALYSIS)
3 Protocols for SiRNA
4 SELEX—in vitro selection
5 Nucleic Acids Research Methods (2)
6 RNA MicroArray Protocol
7 RNA Isolation
Protocols for PCR
1 PCR Based Molecular Cloning
2 Handbook for DNA isolation, RAPD-PCR and PCR-RFLP
3 Introduction to PCR
4 General PCR introduction
5 PCR Animations
6 PCR, RT PCR and Real Time PCR Tutorials
7 PCR Application Manual
8 PCR Elisa
9 PCR RFLP
10 PCR SSCP
11 Real Time PCR
12 RT-PCR Protocols
13 Variants of PCR (2)
14 Variants of PCR (1)
15 AFLP PCR
16 Alu-PCR
17 Asymmetric PCR
18 Colony PCR Protocols
19 Competitive and Quantitative RT-PCR
20 Degenerate PCR
21 Differential Display PCR
22 In Situ PCR
23 Inverse PCR
24 Ligation Mediated Suppression PCR
25 Long PCR Protocols
26 Methylation Specific PCR
27 Multiplex PCR
28 Nested PCR
29 RACE PCR
30 RAPD PCR
31 Rep-PCR
32 TAIL PCR
33 Touchdown PCR
34 Vectorette PCR

Biochemical Resource --(2)

Alzheimer's Disease - Vaccine Prevents Alzheimer's In Mice (applesforhealth)

Basic Biological Structure Revealed (applesforhealth)

Brain - Compound Fights Prion Brain Disease (applesforhealth)

Brain - Probing The Mechanics Of Memory (applesforhealth)

Brain Region Mapped (applesforhealth)

Cancer - Repairing DNA After UV Damage (applesforhealth)

Cancer - Smart Bombs May Blast Cancer Cells (applesforhealth)

Cancer - Unmasking Genetic Causes Of Cancer (applesforhealth)

Cell Transplant Fixes Damaged Brain (applesforhealth)

Biomedia http://ebiomedia.com

The Biology Project: Biochemistry

Chemistry Tutorial

BIOCHEMISTRY TUTORIAL

General, Organic and Biochemistry

www.kumc.edu/research/medicine/biochemistry/bioc800/start.html

AW: Tutorial

Tutorials

biochemistry tutorial, biochemistry online tutorial

Beginning Molecular Biology Laboratory Manual

Biochemistry (Virtual Textbook to accompany Matthews and van Holde edition)

Bioinformatics Courses Around the World

Biol. 2421 Lab Manual - Table of Contents

Cell Biology Laboratory Manual

DNA Isolation Procedures

DNA Purification Protocols and Applications Guide

Electronic Laboratory Manual

ESG Biology Hypertextbook

Kimball's Biology Pages

Medical Biochemistry Subject List

Molecular Biology of the Cell (4th ed)

Molecular Biology Techniques Manual

Molecular Biology Web Book

Molecular Cell Biology (6th ed)

NetBiochem

Online Biology Book

Online Microbiology Lab Manual

Plant Physiology online: A Companion to Plant Physiology, (4th ed)

Principles of Protein Structure Using the Internet

RABINOVITCH PROTOCOL MANUAL

The Science Creative Quarterly, Cell Biology text book page

Western Blotting Handbook and Troubleshooting Guid

Biochemical Resource--(1)

Amino Acid Properties

Beginner's Guide to Molecular Biology

Biochemistry: Course Description

Biochemistry - Web Edition

Biochemistry and Cell Biology

Biochemistry Course Materials

Biochemistry of Neurotransmitters

Biochemistry: Study Guide

Biology Online

The Biology Project: Biochemistry

Biophysical Chemistry

Center for Molecular Modeling

Computational Biochemistry Server at ETHZ

DNA Graphics

European Journal of Biochemistry

Glossary of Biochemistry and Molecular Biology

Graphics Gallery

Images of Proteins/DNA

Kansas University Medical Center Resources for Biochemistry

Life Science Directory

Metabolic Pathways of Biochemistry

Molecular Library

Molecular Models for Biochemistry

NDB Atlas of Nucleic Acid Containing Structures

Cells

Lessons Plans in Science

Biochemistry 1 (BioChemNet - Schmidel)

Biochemistry 2 (BioChemNet - Schmidel)

Biotechnology (BioChemNet - Schmidel)

Biotechnology Dictionary (BioTech Resources)

Cells - Dictionary of Cell and Molecular Biology (Lackey and Dow) 3-00

Genomes Research - Resources (US Department of Energy and the Human Genome Project)

Human Genome Map - Chromosome 21 and Base Pairs (BBC News)

Human Genome Map - Status (Nature - Macmillan Publishers)

Biology Clip Art - Free (BioChemNet - Schmidel)

DNA Sequencing (Howard Hughes Medical Institute)

DNA, Genes, and Heredity (DNA Learning Center)

e

Useful tools and softwares for biochemistry

A comprehensive database of molecular structures
http://www.biomednet.com/db/msd

A biocomputing hypertext coursebook
http://merlin.mbcr.bcm.tmc.edu:8001/bcd/Curric/welcome.html

A molecular graphics art show
http://www.scripps.edu/pub/goodsell/mgs_art/mgs_art.html

A more recent molecular graphics art show
http://www.scripps.edu/pub/goodsell/mgs_art/mgs_art2/index.html

The Protein Data Bank through Microsoft Windows
http://www.sdsc.edu/pb/wpdb/wpdb.htm

Software for biological data representation
http://www.sdsc.edu/pb/Software.html

Home page for a molecular graphics laboratory
http://www.scripps.edu/pub/olson-web/

Hierarchical classification of proteins
http://www.protomap.cs.huji.ac.il/

Biocomputing and molecular modeling
http://www-bio.unizh.ch/

Computational molecular biology at NIH
http://webasaurus.dcrt.nih.gov/molbio/

Online resources for molecular biology
http://webasaurus.dcrt.nih.gov/molbio/servers.html

A collection of biomolecular research tools
http://www.public.iastate.edu/~pedro/research_tools.html

Molecular models for biochemistry
http://info.bio.cmu.edu/Courses/BiochemMols/BCMolecules.html

Image library of biological macromolecules
http://www.imb-jena.de/IMAGE.html

The "Ribbons" software home page
http://www.cmc.uab.edu/ribbons/

PovChem program for molecular graphics
http://grserv.med.jhmi.edu/~paul/Manual.html

The MolMol program for molecular visualization
http://www.mol.biol.ethz.ch/wuthrich/software/molmol/

Molecular biology database compendium
http://www.tulane.edu/~dmsander/biotechniquessites.html

Some biochemistry molecular models
http://moby.ucdavis.edu/HRM/Biochemistry/molecules.htm

A molecular library
http://c4.cabrillo.cc.ca.us/projects/library/

Animated and still biochemistry graphics
http://www-medlib.med.utah.edu/NetBiochem/graphlis.htm

Online macromolecular museum
http://www.clunet.edu/BioDev/omm/gallery.htm

An art gallery of molecular models
http://www2.kenyon.edu/depts/biology/eward/gallery/galleryintro.html

Entrez: Data sets for biochemistry and molecular biology.
http://www3.ncbi.nlm.nih.gov/Entrez/index.html

MolGraph: Molecular modeling program.
http://chemb16.leidenuniv.nl/~armand/

Swiss PDB viewer
http://expasy.expasy.ch/spdbv/

NIH Center for Molecular Modeling
http://cmm.info.nih.gov/modeling/

Virtual reality and biochemistry
http://www.tc.cornell.edu:80/~richard/

Biomolecular Visualization Center
http://amanda.uams.edu/index_text.html

VRML file creator for chemical structures
http://www2.ccc.uni-erlangen.de/services/vrmlcreator/

Rendering techniques for molecular models
http://scsg9.unige.ch/fln/eng/toc.html

The Journal of Molecular Modeling
http://link.springer.de/link/service/journals/00894/index.htm

Using NMR for biomolecular structures
http://www.ocms.ox.ac.uk/idc/structures/

Biomolecular structure and modeling in the United Kingdom
http://www.biochem.ucl.ac.uk/bsm/

Molecular Models Online
http://www.webmolecules.com/index.shtml

Molecular visualization tools and sites
http://www.indiana.edu:80/~cheminfo/mvts.html

RCSB: The home site for all PDB files.
http://www.rcsb.org/pdb/

Biochemistry Protocols Online (part 1)

General Lab Techniques

Lab security and basic techniques

Advanced lab skills-(1)

Advanced lab skills-(2)

Molecular Biology

DNA isolation & related protocols

DNA Purification (glass milk vs electroelution)

DNA and RNA sequencing

Nucleic acid methods (1)

Nucleic acid methods (2)

Isolation of DNA,RNA, and Protein simultaneously.

DNA mutation detection by SSCP

Mouse genotyping by PCR

PCR,RT-PCR,Real time PCR etc.

Southern blot hybridization

Loss of Heterozygosity (LOH)

Gene knockout protocol

RNA Isolation and Purification

Preparation of DNA and RNA probes

Northern blot hybridization

SiRNA gene knockout

Western blot hybridization

Molecular cloning

Conditional gene transfection(Tet on/off)

Protein sequencing

Protein labeling techniques

Subcellular fractionations

EMSA

Methylation interference

Southwestern blotting

Filter binding protocols

Footpring protocols

DNA (RNA) protein interactions

Plasmid and its usefulness

DNA library construction

Microarray protocols.

Protein chips

Methods for detecting protein phosphorylation

Protein methods

Molecular separation

Gene therapy for cancer

X-ray crystallography

Metabolism and chromatographies

GC/MS Background

Glucose metabolism and its related protocols

PAS staining

Polysaccharide sequencing

Deoxyribose procedure

Ribose metabolism analysis

Lactate cleanup and derivative

Amino acids

Fatty acids

Lipid protocols

Cholesterol

Bile acids

Urea procedure

Choline Incorporation Assay

Isotope Ratio Mass Spectrometer (IRMS)

Liquid chromatography / mass spectrometer (LC/MS)

Proteomics

NMR protocols and tutorials

HPLC protocols

TLC

Molecular labeling

Biochemistry Protocols Online (part 2)

Immunology/immunohistochemistry

Preparing silanized (+plus) slides

Microscopy Techniques

Electron microscopy

HE staining

Nucleic acid stain

Special cell & cell fraction stains

Antibody purification

Antibody storage and handling

Conjugation of monoclonal antibodies

Antigen retrieval

Elisa

FRQs for histochemistry

Immunoperoxidase staining techniques

Immunofluorohistochemistry

Confocal microscope technique

Laser Capture Microdissection

Immunoprecipitation

ChIP assay

Hybridization in situ

Histotechnology--technical methods

Flow cytometry (FCM)

Kinase assay

Cellular Biology

General cell culture protocols

Chromosome karyotype

Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)

Cell cycle assay

Migration assay

Stem cell & related protocols

Apoptosis and related protocols

Soft Agar Assay for Colony Formation

Aorta ring assay

GFP transfection

Gene transfection

Transformation protocols

Blood cell fractionation (white blood cell isolation)

Endothelial isolation and culture protocols

Isolation and culture human brain microvessel endothelial cells

Animal Experiments

Blood sampling from animals

Basic skills for animal experiments

Cancer xenograft animal models

Transgenic animal procedures

Transgenic cancer models

Animal search

Animal models for depression-like and anxiety-like behavior

Genetics

Epigenetics protocols

SNPs

Genetic analysis

Mutagenesis

Model Organisms

C. Elegans