Biochemical Protocol.6. Enzymology

Addition of enzyme inhibitors

Advanced analysis of enzyme parameters

Computer simulation

Determination of Km and Vmax

Effects of ph

Effects of temperature

Enzyme Assay（22-28）

Enzyme Assay（36-42）

Enzyme Assay（43-47）

Enzyme concentration

Extraction of tyrosinase

Fucosyltransferase assay

Introduction

Kinetic analysis

Phosphatase assay

Preparation of standard curve

Protein concentration/enzyme activity

Biochemical Protocol.7. RNA

Amino-allyl labeling

BioAnalyzer
TRIzol plus RNEasy Mini
RNEasy Midi
TRIzol product insert
RNA amplification
RNA gel electrophoresis

C. elegans RNA prep: Michael Koelle

In vitro transcription reaction

Isolation of poly-A RNA

LiCl RNA Preparation: MGH

Northern blots

Northern Blots: Michael Koelle

Nucleotide composition of RNA

Orcinol determination of RNA

Phenol extraction of rRNA (rat liver)

RNA and protein extraction from the same sample

RNA prepararion (from plants tissue)

RNA Preparation: MGH

Spectrophotometric analysis of RNA

Sucrose density fractionation

Worm RNA Preparation: MGH

Biochemical Protocol.8. DNA (1)

ABC Random Amplification
Alcohol Precipitation of DNA
AML sample DNA preparation

Agarose gel electrophoresis

Agarose gel electrophoresis

Boomerang DNA Amplification Single primer DNA amplification

cDNA amplification from l-phage library

cDNA library screening

Characterization of DNA

DIG DNA labelling and hybrid detection (Boehringer Mannheim)

Direct Automated Sequencing off Bacterial Genomic DNABruce Rhoe

DNA extraction from agarose (Qiagen)

DNA - dische diphenylamine determination

DNA Fragment Isolation from LM agarose

DNA Ligation

DNA detection
DAPI chromosome identification

DNA preparation by cryostom tissue dissection
DNA labeling by nick translation

Ethanol precipitation of DNA

Extraction of DNA from bovine spleen

Fluorometry
FISH

Biochemical Protocol.8. DNA (2)

Genomic DNA preparation (plants)
Hybridization

Large Scale Plasmid Preparation: MGH

Modifications of DNA ends

PCR amplification of DNA

PCR product purification (Qiagen)

Perfectly Blunt Cloning of DNA (Novagen)

Phenol/chloroform extraction of DNA

Plasmid midi-prep from bacteria

Preparation of genomic DNA from bacteria

Purification of DNA

Q-PCR

Random Primed labeling of DNA (Prime-It II -Stratagene)

Restriction enzyme digestion of DNA
Southern Hybridization
Stark's Prehybridization Buffer

Sequencing using Sequenase

Southern blot

Tail DNA prep

Wizard Plus Mini-preps (109. Plasmid Mini-prep)

Worm Genomic DNA: MGH

Worm genomic Southern blots: Michael Koelle

In vivo transcription

BrUTP labeling of RNA transcripts

Biochemical Protocol.9. PCR (1)

About PCR

AFLP PCR

Alu-PCR

Asymmetric PCR

Colony PCR Protocols

Competitive and/or Quantitative RT-PCR

Degenerate PCR

Differential Display PCR

Genotyping by PCR

History of Polymerase chain reaction (PCR)--(1)

History of Polymerase chain reaction (PCR)--(2)

Important Publications at Cell

Important Publications at Nature

Important Publications at Science

In Situ PCR

Introduction to PCR

Inverse PCR

Ligation Mediated Suppression PCR

Long PCR Protocol: Church Lab

Long PCR Protocols

Methylation Specific PCR

Multiplex PCR

Nested PCR

PCR Animations

PCR aplications

PCR Brochure and Articles

PCR Elisa

PCR Manual

PCR Primer Design Tools

Biochemical Protocol.9. PCR (2)

PCR RFLP

PCR SSCP

PCR Troubleshooting

PCR, RT-PCR, and Real Time PCR Tutorials

Polymerase Chain Reaction (Molecular Info)

Polymerase chain reaction--PCR

RACE PCR

RAPD PCR

REAL TIME PCR Papers

Real Time PCR Protocols

Regular PCR Procedure

REP-PCR

RT PCR Protocols

Special PCR Protocols

Tail PCR

Tc1 Deletion PCR Tips: MGH

Touchdown PCR

Variants of PCR (1)

Variants of PCR (2)

Variants of PCR (3)

Variants of PCR (4)

Variants of PCR (5)

Vectorette PCR

Videos and Animations for PCR

Web Source of PCR (1)

Web Source of PCR (2)

Web Source of PCR (3)

Web Source of PCR (3)

Biochemical Protocol.10. Cellular Biology

Aseptic Cell Transfers

Calcium Phosphate tranfection of adherent mammalian cells (works well for 293 cells)

Cell mass by Measurement of Turbidity

Computation of Transfer Aliquots

Culturing MEFs
Calcium Phosphate Coprecipitation

Determining cell counts

Effectene (QIAGEN) tranfection of adherent mammalian cells (works well for HeLas and many other cell types)

Establishing stable cell lines

Establishment of a Primary Culture

Eukaryote Growth Dynamics

Examination of Bacterial Colonies

Fugene (Roche) tranfection of adherent mammalian cellsDOTAP (Roche) tranfection of adherent mammalian cells

Fusing cells to create heterokaryons

Gram Stain (+\-)

Harvesting murine bone marrow

Hematopoietic CFC assays

Maintenance

Metaphase chromosome preparation

Preservation and storage

Prokaryote Cell Number by Dilution Plating

Purification of L-KS+ marrow cells
Retrovirus Production
STO and ES Cell Culture

Safety considerations

Tissue culture methods

Tissue Culture of PtK1 Cells

Transfer of Eukaryote Suspension Cultures

TUNEL Protocol

Types of cells grown in culture

Viability Cell Count

Work area and equipment
XC assay of MoMLV