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Friday, December 12, 2008

Biochemical Protocol.6. Enzymology

Addition of enzyme inhibitors

Advanced analysis of enzyme parameters

Computer simulation

Determination of Km and Vmax

Effects of ph

Effects of temperature

Enzyme Assay22-28

Enzyme Assay36-42

Enzyme Assay43-47

Enzyme concentration

Extraction of tyrosinase

Fucosyltransferase assay

Introduction

Kinetic analysis

Phosphatase assay

Preparation of standard curve

Protein concentration/enzyme activity

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