MT/Organelle Motility Assay*
Membrane Mixes: Variations on a Theme*
Preparation of Rat Liver Cell Cytosol and/or Organelle Fractions*
Preparation of Sea Urchin Sperm Axonemes*
Preparation of Porcine Brain Tubulin*
Friday, December 12, 2008
Biochemical Protocol.11. Cellular Organelle
Biochemical Protocol.12. Immunology
Antibody cleanup
Bioengineering and Immunology (Video Protocol)
Conjugation of monoclonal antibodies (complete) Dr. M Roederer, NIH,
Direct Immunofluorescence Staining of Mononuclear Cells BD Biosciences
Direct Immunofluorescence Staining of Whole Blood Using Ammonium Chloride Lysing Solution BD Biosciences
Iindirect immunoflourescence for yeast I Dr. DE Koshland, Carnegie Institution of Washington
Immunofluorescence Protocols and Solutions
Immunofluorescence staining of cell suspensions of murine lymphoid tissue EBioscience.com
Immunofluorescence staining of human peripheral blood EBioscience.com
Immunofluorescence Technique Dr. DL Spector,
Immunofluorescent Staining of Mouse and Rat Leucocytes BD Biosciences
Immunohistochemistry on Cryosections
Immunology Field Trip--Protocol
Indirect Immunofluorescence Staining of Mononuclear Cells BD Biosciences
Isolation of Resting B Cells From Mouse Spleen
MACS Depletion Drs. L Herzenberg,
Making FITC and Biotin conjugates Therapeutic Immunology Group,
Preparing cells for immunofluorescence
Biochemical Protocol.13. Flow Cytometry Protocols (1)
7-AAD Staining of Dead Cells
A flow cytometric protocol for detection of Cryptosporidium spp.
Calibrating Becton Dickinson Flow Cytometers for RFI Measurements
CD38 Fluorescence Intensity Quantitation
Detection of Apoptosis by 7-AAD Staining
DNA Staining for Cell Cycle Analysis
FCM: DNA Analysis by Flow Cytometry
FCM: FASC Analysis of Cell Cycle using BrdU and PI
FCM: Immunofluorescent Staining of Rat Thymocytes
FCM: Sodium and Potassium Analysis by Flow Cytometry
Flow cytometry of murine blood
Flow Cytometry Principles （UC Berkeley）
Flow Cytometry Protocol for Intracellular Staining Using Conjugated Second Antibody.
Flow Cytometry Protocol for Staining of Colonocytes - Surface Antigens
Flow Cytometry: Calcium Flux Analysis
Flow Cytometry: Membrane Potential Analysis by Flow Cytometry
Flow Cytometry：Annexin V Protocol
Flow Cytometry：CaspaTag Caspase Activity Protocol
Biochemical Protocol.13. Flow Cytometry Protocols (2)
Immunofluorescent Staining for Flow Cytometry.
Indirect flow cytometry (FACS) protocol | Abcam
Intracellular Antigen Staining
Lineage analysis of blood
MEF nuclei prep for DNA content
Flow of surface and nuclear markers
BrdU and PI Staining Cells for Flow
Simultaneous BrdU and surface staining
Flow sorting fibroblasts for GFP
Setup and Use of the Cytometer
96-well Flow Setup Record (PDF)
Counting Fluorescently Stained Cells
Measurement of GFP Expression and DNA Content in Permeabilized Cells
Measurement of Green Fluorescent Protein Expression and DNA Content in Unfixed Cells
Measurement of Intracellular CA2+ and Cell Surface Antigen Expression -- NEW!
Method for EtOH fixation of cells for long-term storage and DNA staining
Minimizing Background Staining
Monoclonal Antibody Staining Procedure
Preparation of 2% Formaldehyde Solution
Propidium Iodide Staining of Dead Cells
Protocol: Flow Cytometry (Millipore)
Protocols (BD Bioscience)
Questions and Answers about Cell Sorting
Simultaneous Measurement of Apoptosis and Necrosis
Simultaneous Measurement of Cell Surface Immunofluorescence, Viability, and DNA content
Simultaneous Measurement of Dual-Color Immunofluorescence and DNA/RNA Content
Simultaneous Measurement of Three-Color Immunofluorescence and DNA Content
Staining Procedure for Whole Blood
Standard Flow Cytometry Protocol
Biochemical Protocol.15. BACTERIA
Electro Competent Agrobacterium Cells
Introduction of plasmid into Agrobacterium (method II)
Introduction of plasmid into Agrobacterium (method III)
General Cloning Procedures
Colony Hybridization
Electroporation & Blue/White Selection
Large scale plasmid prep, PEG method
Large scale plasmid prep, Qiagen/CsCl
Ligation Optimization
Plasmid Miniprep
Preparation of competent E. coli cells using CaCl2
Transformation of bacteria
Release of recombinant proteins from bacterial cells using Novagen's Bugbuster
Biochemical Protocol.16. Arabidopsis Protocols
Arabidopsis cell suspension and root liquid culture
Arabidopsis EMS mutagenesis of seeds
Arabidopsis genomic DNA prep-CTAB extraction method
Arabidopsis genomic DNA prep-Proteinase K method
Arabidopsis quick genomic DNA prep for PCR
Arabidopsis seed sterilization
Arabidopsis vacuum infiltration (plant transformation)
Confocal Microscopy- Nuclear Staining
Cross-fertilization of Arabidopsis
Crossing Arabidopsis plants (flower emmansculation and flower preparation for fertilization)
Glucocorticoid (GVG)-inducible promoter system for Arabidopsis
In situ hybridization using radioactive probes
Non radioactive in situ hybridization
Plastic Sectioning- Tissue fixation
Protoplast preparation (plant tissue)
Scanning Electron Microscope- Tissue fixation and dissection
Subscribe to:
Comments (Atom)
Posts
