Biochemical Protocol.13. Flow Cytometry Protocols (2)

Immunofluorescent Staining for Flow Cytometry.

Indirect flow cytometry (FACS) protocol | Abcam

Intracellular Antigen Staining

Lineage analysis of blood
MEF nuclei prep for DNA content
Flow of surface and nuclear markers
BrdU and PI Staining Cells for Flow
Simultaneous BrdU and surface staining
Flow sorting fibroblasts for GFP
Setup and Use of the Cytometer
96-well Flow Setup Record (PDF)
Counting Fluorescently Stained Cells

Measurement of GFP Expression and DNA Content in Permeabilized Cells

Measurement of Green Fluorescent Protein Expression and DNA Content in Unfixed Cells

Measurement of Intracellular CA2+ and Cell Surface Antigen Expression -- NEW!

Method for EtOH fixation of cells for long-term storage and DNA staining

Minimizing Background Staining

Monoclonal Antibody Staining Procedure

Preparation of 2% Formaldehyde Solution

Propidium Iodide Staining of Dead Cells

Protocol: Flow Cytometry (Millipore)

Protocols (BD Bioscience)

Questions and Answers about Cell Sorting

Simultaneous Measurement of Apoptosis and Necrosis

Simultaneous Measurement of Cell Surface Immunofluorescence, Viability, and DNA content

Simultaneous Measurement of Dual-Color Immunofluorescence and DNA/RNA Content

Simultaneous Measurement of Three-Color Immunofluorescence and DNA Content

Staining Procedure for Whole Blood

Standard Flow Cytometry Protocol

Surface Antigen Staining and DNA Content

Surface Staining for Cell Sorting