Biochemical Protocol.13. Flow Cytometry Protocols (1)

7-AAD Staining of Dead Cells

A flow cytometric protocol for detection of Cryptosporidium spp.

Blocking FC-Receptor Binding

Calibrating Becton Dickinson Flow Cytometers for RFI Measurements

CD38 Fluorescence Intensity Quantitation

Detection of Apoptosis by 7-AAD Staining

DNA Staining for Cell Cycle Analysis

FCM: DNA Analysis by Flow Cytometry

FCM: FASC Analysis of Cell Cycle using BrdU and PI

FCM: Immunofluorescent Staining of Rat Thymocytes

FCM: Sodium and Potassium Analysis by Flow Cytometry

Flow Cytometry Glossary

Flow cytometry of murine blood

Flow Cytometry Principles （UC Berkeley）

Flow Cytometry Protocol

Flow Cytometry Protocol for Intracellular Staining Using Conjugated Second Antibody.

Flow Cytometry Protocol for Staining of Colonocytes - Surface Antigens

Flow Cytometry: Calcium Flux Analysis

Flow Cytometry: Membrane Potential Analysis by Flow Cytometry

Flow Cytometry：Annexin V Protocol

Flow Cytometry：CaspaTag Caspase Activity Protocol

Flow Cytometry：PARP PE

GENERAL CELL STAINING PROTOCOL FOR FLOW CYTOMETRY 1)

Biochemical Protocol.13. Flow Cytometry Protocols (2)

Immunofluorescent Staining for Flow Cytometry.

Indirect flow cytometry (FACS) protocol | Abcam

Intracellular Antigen Staining

Lineage analysis of blood
MEF nuclei prep for DNA content
Flow of surface and nuclear markers
BrdU and PI Staining Cells for Flow
Simultaneous BrdU and surface staining
Flow sorting fibroblasts for GFP
Setup and Use of the Cytometer
96-well Flow Setup Record (PDF)
Counting Fluorescently Stained Cells

Measurement of GFP Expression and DNA Content in Permeabilized Cells

Measurement of Green Fluorescent Protein Expression and DNA Content in Unfixed Cells

Measurement of Intracellular CA2+ and Cell Surface Antigen Expression -- NEW!

Method for EtOH fixation of cells for long-term storage and DNA staining

Minimizing Background Staining

Monoclonal Antibody Staining Procedure

Preparation of 2% Formaldehyde Solution

Propidium Iodide Staining of Dead Cells

Protocol: Flow Cytometry (Millipore)

Protocols (BD Bioscience)

Questions and Answers about Cell Sorting

Simultaneous Measurement of Apoptosis and Necrosis

Simultaneous Measurement of Cell Surface Immunofluorescence, Viability, and DNA content

Simultaneous Measurement of Dual-Color Immunofluorescence and DNA/RNA Content

Simultaneous Measurement of Three-Color Immunofluorescence and DNA Content

Staining Procedure for Whole Blood

Standard Flow Cytometry Protocol

Surface Antigen Staining and DNA Content

Surface Staining for Cell Sorting

Biochemical Protocol.14. BIOCHEMISTRY

Cell Wall prep from Arabidopsis

GC analysis of alditol acetates

Golgi-Vesicles preparation from pea etiolated hypocotyls

High Voltage Paper Electrophoresis

Lipid extraction

Thin Layer Chromatography

Transport of UDP-[3H]-Glc to Golgi-Vesicles

UDP-sugar binding assay

Biochemical Protocol.15. BACTERIA

Electro Competent Agrobacterium Cells

Electroporation

Introduction of plasmid into Agrobacterium (method II)

Introduction of plasmid into Agrobacterium (method III)
General Cloning Procedures
Colony Hybridization
Electroporation & Blue/White Selection
Large scale plasmid prep, PEG method
Large scale plasmid prep, Qiagen/CsCl
Ligation Optimization
Plasmid Miniprep

Preparation of competent E. coli cells using CaCl2

Transformation of bacteria
Release of recombinant proteins from bacterial cells using Novagen's Bugbuster

Purification of recombinant hexahistidine-tagged proteins

Purification of recombinant GST-tagged proteins

Biochemical Protocol.16. Arabidopsis Protocols

Arabidopsis cell suspension and root liquid culture

Arabidopsis EMS mutagenesis of seeds

Arabidopsis genomic DNA prep-CTAB extraction method

Arabidopsis genomic DNA prep-Proteinase K method

Arabidopsis GUS staining

Arabidopsis liquid culture

Arabidopsis mutants

Arabidopsis quick genomic DNA prep for PCR

Arabidopsis root culture

Arabidopsis seed sterilization

Arabidopsis vacuum infiltration (plant transformation)

Confocal Microscopy- Nuclear Staining

Cross-fertilization of Arabidopsis

Crossing Arabidopsis plants (flower emmansculation and flower preparation for fertilization)

Glucocorticoid (GVG)-inducible promoter system for Arabidopsis

How we grow Arabidopsis

In situ hybridization using radioactive probes

Non radioactive in situ hybridization

Plastic Sectioning- Tissue fixation

Protoplast preparation (plant tissue)

Scanning Electron Microscope- Tissue fixation and dissection

Transmission Electron Microscopy- Tissue fixation

Vacuum infiltration of Arabidopsis plants

Biochemical Protocol.17. Yeast Protocols (1)

96-Well Format Yeast Protein Purification Protocol

A microarray-based protocol for monitoring the growth of yeast .

A protocol for isolation and visualization of yeast nuclei by scanning electron microscopy (SEM).

BLAST Searching from SGD.

Brent Lab Two-Hybrid Information

Chromatin immunoprecipitation protocol for Saccharomyces cerevisiae.

Codon Usage Tables.

Colony PCR

Common Access to Biological Resources and Information (CABRI)

Commonly used lab strains

Competent Yeast Preparation

Confirmation PCR

Direct PCR from Whole Yeast Cells

DNA Replication Protocols from the Fangman and Brewer Labs, University of Washington.

DNA Replication Techniques

European announcement of the complete Sequence, April 24, 1996.

FASTA
S. cerevisiae protein structural information
Yeast Maps
Genomic View
Worm-Yeast Protein Comparison
Mammalian Homology to Yeast
Yeast Genome Restriction Analysis
Search Saccharomyces GenBank Sequences
Pairwise Chromosome Similarity View

FASTA Searching from SGD.

Gene Disruption via PCR
Confirmation PCR

GeneQuiz - Consortium for large scale sequence analysis.

Gietz Lab's Yeast Transformation Page

Growth Chamber for Time Lapse Imaging of Budding Yeast

GST Fusion Protein Purification from Yeast

Interaction Trap at Work

INTESTINAL DYSBIOSIS / YEAST PROTOCOL

List of species specific documents from SWISS-PROT.

National Collection of Yeast Cultures (NCYC)

NCRR Yeast Resource Center

Paul's Yeast CHIP

PCR to check genotype

Plasmid Rescue from Yeast from Liquid Culture and Clony Rescue

Preparation of Yeast Cell for Immunoeletron Microscopy

Protein Prep

Protein Search from MIPS.

Protocol for Yeast-Based Androgen Receptor Assay ...

Quick Total Yeast Protein Isolation for SDS-PAGE Gels

Biochemical Protocol.17. Yeast Protocols (2)

Saccharomyces Gene Registry at SGD.

Slide Stripping Protocol Agilent Yeast Arrays

Staining of yeast by Rhodamine-Phalloidin/Calcofluor

Sucrose Density Gradient Fractionation of Yeast Membranes

Systematic Sequencing Table f

The Definitive Yeast Transformation Homepage from the University of Manitoba.

The MGuide. Version 2.0

The World-Wide Web Virtual Library: Yeast

Tritech Research

VectorDB, collected sequences of cloning vectors for use in a variety of organisms.

Vectorette PCR of Yeast DNA

Whole Cell Extracts

Yale Genome Analysis Center

Yeast Actin Capture Assay

Yeast Actin Purification protocol

Yeast Cell Cycle Flow Cytometry Protocol

Yeast Cell Micromanipulation, a protocol for making a support rod with fiber optic needle attached.

Yeast Colony PCR

Yeast Genome Directory

Yeast Genome Project Information from MIPS.

Yeast GST-fusion Collection

Yeast Histone Prep (from Jakob Waterborg; .pdf format)

Yeast IF without Dehydration

Yeast Immunofluorescence protocol

Yeast Immunofluorescence with Methanol/Acetone Dehydration

Yeast Immunoprecipitation

Yeast Knockout (YKO) Collection

Yeast Lab Protocols from the Botstein Lab, Stanford University.

Yeast Lab Protocols from the Breeden Lab, Fred Hutchinson Cancer Research Center.

Yeast Lab Protocols from the Gottschling Lab, Fred Hutchinson Cancer Research Center.

Yeast Mitochondrial Protein Database

Yeast Nuclear Extract Prep (small scale)

Yeast Protein Preparation for SDS PAGE and Western (rapid)

Yeast Proteome Database (YPD), accessible only to subscribers.

Yeast ProtoArrayTM Proteome Chips

Yeast Protocols

Yeast Protocols from the Gottschling Lab

Yeast Protocols Handbook

Yeast Sequencing at the Sanger Centre.

Yeast Sporulation

Yeast Tet-promoters Hughes Collection

Yeast Transposon Insertion Library Collection

Yeast Two-Hybrid Analisys

Yeast Whole Cell Extract (small scale)

Yeast-GFP Clone Collection

Yeast-TAP Fusion Library.