Review Articles on Embryonic Stem Cell Research (1)

1.

Cardiac fibroblast: the renaissance cell.

Souders CA, Bowers SL, Baudino TA.

Circ Res. 2009 Dec 4;105(12):1164-76. Review.PMID: 19959782 [PubMed - indexed for MEDLINE]Related articles

2.

The use of SC1 (Pluripotin) to support mESC self-renewal in the absence of LIF.

Xiong W, Gao Y, Cheng X, Martin C, Wu D, Yao S, Kim MJ, Liu Y.

J Vis Exp. 2009 Nov 18;(33). pii: 1550. doi: 10.3791/1550.PMID: 19924098 [PubMed - indexed for MEDLINE]Related articlesFree article

3.

Generation of induced pluripotent stem cells by reprogramming mouse embryonic fibroblasts with a four transcription factor, doxycycline inducible lentiviral transduction system.

Hamilton B, Feng Q, Ye M, Welstead GG.

J Vis Exp. 2009 Nov 13;(33). pii: 1447. doi: 10.3791/1447.PMID: 19915522 [PubMed - indexed for MEDLINE]Related articlesFree article

4.

Multipotent skin-derived precursors: from biology to clinical translation.

Hunt DP, Jahoda C, Chandran S.

Curr Opin Biotechnol. 2009 Oct;20(5):522-30. Epub 2009 Nov 5. Review.PMID: 19896826 [PubMed - indexed for MEDLINE]Related articles

5.

Skeletal morphogenesis during embryonic development.

Yang Y.

Crit Rev Eukaryot Gene Expr. 2009;19(3):197-218. Review.PMID: 19883365 [PubMed - indexed for MEDLINE]Related articles

6.

Genome modification in human embryonic stem cells.

Tenzen T, Zembowicz F, Cowan CA.

J Cell Physiol. 2010 Feb;222(2):278-81. Review.PMID: 19877154 [PubMed - indexed for MEDLINE]Related articles

7.

Isolation and derivation of mouse embryonic germinal cells.

Moreno-Ortiz H, Esteban-Perez C, Badran W, Kent-First M.

J Vis Exp. 2009 Oct 22;(32). pii: 1635. doi: 10.3791/1635.PMID: 19851276 [PubMed - indexed for MEDLINE]Related articlesFree article

8.

The role of Runx1/AML1 and Evi-1 in the regulation of hematopoietic stem cells.

Kumano K, Kurokawa M.

J Cell Physiol. 2010 Feb;222(2):282-5. Review.PMID: 19847803 [PubMed - indexed for MEDLINE]Related articles

9.

Molecular events in germ cell tumours: linking chromosome-12 gain, acquisition of pluripotency and response to cisplatin.

Korkola JE, Houldsworth J, Bosl GJ, Chaganti RS.

BJU Int. 2009 Nov;104(9 Pt B):1334-8. Review.PMID: 19840009 [PubMed - indexed for MEDLINE]Related articles

10.

Potential application of adult stem cells in retinal repair--challenge for regenerative medicine.

Machalińska A, Baumert B, Kuprjanowicz L, Wiszniewska B, Karczewicz D, Machaliński B.

Curr Eye Res. 2009 Sep;34(9):748-60. Review.PMID: 19839868 [PubMed - indexed for MEDLINE]Related articles

11.

Gene silencing in human embryonic stem cells by RNA interference.

Rassouli FB, Matin MM.

Biochem Biophys Res Commun. 2009 Dec 25;390(4):1106-10. Epub 2009 Oct 13. Review.PMID: 19833094 [PubMed - indexed for MEDLINE]Related articles

12.

Auxin gradients trigger de novo formation of stem cells during somatic embryogenesis.

Su YH, Zhang XS.

Plant Signal Behav. 2009 Jul;4(7):574-6. Epub 2009 Jul 15. Review.PMID: 19820347 [PubMed - indexed for MEDLINE]Related articlesFree article

13.

Gene expression in stem cells.

Liang Y, Russell I, Walworth C, Chen C.

Crit Rev Eukaryot Gene Expr. 2009;19(4):289-300. Review.PMID: 19817706 [PubMed - indexed for MEDLINE]Related articles

14.

Regulation of stem cell pluripotency and neural differentiation by lysophospholipids.

Pitson SM, Pébay A.

Neurosignals. 2009;17(4):242-54. Epub 2009 Sep 30. Review.PMID: 19816061 [PubMed - indexed for MEDLINE]Related articles

15.

Signals involved in neural differentiation of human embryonic stem cells.

Denham M, Dottori M.

Neurosignals. 2009;17(4):234-41. Epub 2009 Sep 30. Review.PMID: 19816060 [PubMed - indexed for MEDLINE]Related articles

16.

Mesenchymal stem cells for bone repair and metabolic bone diseases.

Undale AH, Westendorf JJ, Yaszemski MJ, Khosla S.

Mayo Clin Proc. 2009 Oct;84(10):893-902. Review.PMID: 19797778 [PubMed - indexed for MEDLINE]Related articles

17.

Complex and context dependent regulation of hematopoiesis by TGF-beta superfamily signaling.

Söderberg SS, Karlsson G, Karlsson S.

Ann N Y Acad Sci. 2009 Sep;1176:55-69. Review.PMID: 19796233 [PubMed - indexed for MEDLINE]Related articles

18.

The migration of autonomic precursor cells in the embryo.

Kulesa PM, Lefcort F, Kasemeier-Kulesa JC.

Auton Neurosci. 2009 Nov 17;151(1):3-9. Epub 2009 Sep 23. Review.PMID: 19783486 [PubMed - indexed for MEDLINE]Related articles

19.

Making a neuron: Cdk5 in embryonic and adult neurogenesis.

Jessberger S, Gage FH, Eisch AJ, Lagace DC.

Trends Neurosci. 2009 Nov;32(11):575-82. Epub 2009 Sep 24. Review.PMID: 19782409 [PubMed - indexed for MEDLINE]Related articles

20.

Stem cells and somatic cells: reprogramming and plasticity.

Estrov Z.

Clin Lymphoma Myeloma. 2009;9 Suppl 3:S319-28. Review.PMID: 19778860 [PubMed - indexed for MEDLINE]Related articles

21.

Derivation of insulin-producing cells from human embryonic stem cells.

Van Hoof D, D'Amour KA, German MS.

Stem Cell Res. 2009 Sep-Nov;3(2-3):73-87. Epub 2009 Aug 26. Review.PMID: 19766074 [PubMed - indexed for MEDLINE]Related articles

22.

Skeletal muscle stem cells in developmental versus regenerative myogenesis.

Tajbakhsh S.

J Intern Med. 2009 Oct;266(4):372-89. Review.PMID: 19765181 [PubMed - indexed for MEDLINE]Related articles

23.

Induced pluripotent stem cells in regenerative medicine: an argument for continued research on human embryonic stem cells.

Lee H, Park J, Forget BG, Gaines P.

Regen Med. 2009 Sep;4(5):759-69. Review.PMID: 19761400 [PubMed - indexed for MEDLINE]Related articles

24.

Engineering microenvironments for embryonic stem cell differentiation to cardiomyocytes.

Horton RE, Millman JR, Colton CK, Auguste DT.

Regen Med. 2009 Sep;4(5):721-32. Review.PMID: 19761397 [PubMed - indexed for MEDLINE]Related articles

25.

Molecular regulation of vertebrate retina cell fate.

Andreazzoli M.

Birth Defects Res C Embryo Today. 2009 Sep;87(3):284-95. Review.PMID: 19750521 [PubMed - indexed for MEDLINE]Related articles

26.

Progenitor cell therapy for heart disease.

Gonzales C, Pedrazzini T.

Exp Cell Res. 2009 Nov 1;315(18):3077-85. Epub 2009 Sep 10. Review.PMID: 19747911 [PubMed - indexed for MEDLINE]Related articles

27.

Induced pluripotent stem cells from swine (Sus scrofa): why they may prove to be important.

Roberts RM, Telugu BP, Ezashi T.

Cell Cycle. 2009 Oct 1;8(19):3078-81. Epub 2009 Oct 21. Review.PMID: 19738434 [PubMed - indexed for MEDLINE]Related articles

28.

Chromosomal spread preparation of human embryonic stem cells for karyotyping.

Campos PB, Sartore RC, Abdalla SN, Rehen SK.

J Vis Exp. 2009 Sep 4;(31). pii: 1512. doi: 10.3791/1512.PMID: 19734841 [PubMed - indexed for MEDLINE]Related articlesFree article

29.

Stem cells, stress, metabolism and cancer: a drama in two Octs.

Kang J, Shakya A, Tantin D.

Trends Biochem Sci. 2009 Oct;34(10):491-9. Epub 2009 Sep 4. Review.PMID: 19733480 [PubMed - indexed for MEDLINE]Related articles

30.

Cancer stem cell genomics: the quest for early markers of malignant progression.

Okamoto OK.

Expert Rev Mol Diagn. 2009 Sep;9(6):545-54. Review.PMID: 19732002 [PubMed - indexed for MEDLINE]Related articles

31.

Molecular and cellular determinants for generating ES-cell derived dopamine neurons for cell therapy.

Pruszak J, Isacson O.

Adv Exp Med Biol. 2009;651:112-23. Review.PMID: 19731556 [PubMed - indexed for MEDLINE]Related articles

32.

Notch signalling and haematopoietic stem cell formation during embryogenesis.

Gering M, Patient R.

J Cell Physiol. 2010 Jan;222(1):11-6. Review.PMID: 19725072 [PubMed - indexed for MEDLINE]Related articles

33.

Stem cell-related therapies for vascular diseases.

Martin-Rendon E, Snowden JA, Watt SM.

Transfus Med. 2009 Aug;19(4):159-71. Review.PMID: 19706133 [PubMed - indexed for MEDLINE]Related articles

34.

Nuclear receptor regulation of stemness and stem cell differentiation.

Jeong Y, Mangelsdorf DJ.

Exp Mol Med. 2009 Aug 31;41(8):525-37. Review.PMID: 19696553 [PubMed - indexed for MEDLINE]Related articlesFree article

35.

Cardiac applications for human pluripotent stem cells.

Shiba Y, Hauch KD, Laflamme MA.

Curr Pharm Des. 2009;15(24):2791-806. Review.PMID: 19689350 [PubMed - indexed for MEDLINE]Related articles

36.

The role of Krüppel-like factors in the reprogramming of somatic cells to induced pluripotent stem cells.

Nandan MO, Yang VW.

Histol Histopathol. 2009 Oct;24(10):1343-55. Review.PMID: 19688699 [PubMed - indexed for MEDLINE]Related articlesFree article

37.

In vivo fate tracing studies of mammalian taste cell progenitors.

Thirumangalathu S, Barlow LA.

Ann N Y Acad Sci. 2009 Jul;1170:34-8. Review.PMID: 19686103 [PubMed - indexed for MEDLINE]Related articlesFree article

38.

Cancer stem cells: the other face of Janus.

Mittal S, Mifflin R, Powell DW.

Am J Med Sci. 2009 Aug;338(2):107-12. Review.PMID: 19680015 [PubMed - indexed for MEDLINE]Related articles

39.

Emerging concepts in neural stem cell research: autologous repair and cell-based disease modelling.

Koch P, Kokaia Z, Lindvall O, Brüstle O.

Lancet Neurol. 2009 Sep;8(9):819-29. Review.PMID: 19679274 [PubMed - indexed for MEDLINE]Related articles

40.

New complexity in differentiating stem cells toward hepatic and pancreatic fates.

Huppert SS, Magnuson MA.

Sci Signal. 2009 Aug 11;2(83):pe50. Review.PMID: 19671927 [PubMed - indexed for MEDLINE]Related articles

41.

Deciphering the stem cell machinery as a basis for understanding the molecular mechanism underlying reprogramming.

Bosnali M, Münst B, Thier M, Edenhofer F.

Cell Mol Life Sci. 2009 Nov;66(21):3403-20. Epub 2009 Aug 7. Review.PMID: 19662495 [PubMed - indexed for MEDLINE]Related articlesFree article

42.

Toward clinical therapies using hematopoietic cells derived from human pluripotent stem cells.

Kaufman DS.

Blood. 2009 Oct 22;114(17):3513-23. Epub 2009 Aug 3. Review.PMID: 19652198 [PubMed - indexed for MEDLINE]Related articles

43.

Stem/progenitor cells in liver injury repair and regeneration.

Zhao Q, Ren H, Zhu D, Han Z.

Biol Cell. 2009 Jul 31;101(10):557-71. Review.PMID: 19642968 [PubMed - indexed for MEDLINE]Related articles

44.

Dissection of gene regulatory networks in embryonic stem cells by means of high-throughput sequencing.

Beisel C, Paro R.

Biol Chem. 2009 Nov;390(11):1139-44. Review.PMID: 19642873 [PubMed - indexed for MEDLINE]Related articles

45.

Current progress and prospects of induced pluripotent stem cells.

Chen L, Liu L.

Sci China C Life Sci. 2009 Jul;52(7):622-36. Epub 2009 Jul 30. Review.PMID: 19641867 [PubMed - indexed for MEDLINE]Related articles

46.

Generation of pancreatic islet cells from human embryonic stem cells.

Zhang D, Jiang W, Shi Y, Deng H.

Sci China C Life Sci. 2009 Jul;52(7):615-21. Epub 2009 Jul 30. Review.PMID: 19641866 [PubMed - indexed for MEDLINE]Related articles

47.

Human parthenogenetic embryonic stem cells: one potential resource for cell therapy.

Hao J, Zhu W, Sheng C, Yu Y, Zhou Q.

Sci China C Life Sci. 2009 Jul;52(7):599-602. Epub 2009 Jul 30. Review.PMID: 19641863 [PubMed - indexed for MEDLINE]Related articles

48.

Embryonic stem cell models in vascular biology.

Li X, Claesson-Welsh L.

J Thromb Haemost. 2009 Jul;7 Suppl 1:53-6. Review.PMID: 19630768 [PubMed - indexed for MEDLINE]Related articles

49.

Progenitor cell trafficking in the vascular wall.

Hristov M, Weber C.

J Thromb Haemost. 2009 Jul;7 Suppl 1:31-4. Review.PMID: 19630763 [PubMed - indexed for MEDLINE]Related articles

50.

Stem cells, pluripotency and nuclear reprogramming.

Jaenisch R.

J Thromb Haemost. 2009 Jul;7 Suppl 1:21-3. Review.PMID: 19630760 [PubMed - indexed for MEDLINE]Related articles

Viral Protocols and Protocol Links (2)

Paramyxoviruses - http://en.wikipedia.org/wiki/Paramyxovirus
Information from Wikipedia on these negative-sense single-stranded RNA viruses, responsible for a number of human and animal diseases, including their taxonomy, morphology and pathogenesis.

Poxvirus Bioinformatics Resource - http://www.poxvirus.org
The Poxvirus Bioinformatics Resource Center has been established to provide resources to the scientific community for basic research and to facilitate the development of novel antiviral therapies and vaccines against human orthopoxvirus infections as well as approaches for environmental detection of virions and the rapid diagnosis of disease.

Viral Replication - http://en.wikipedia.org/wiki/Viral_replication
Information from Wikipedia on the attachment, entry, and expression of viral genomic material in host cells.

Wadsworth Center - New York State Department of Health - http://www.wadsworth.org/databank/viruses.htm
Information and images regarding; Rabies virus, Herpes virus, Enteroviruses, Norwalk virus, Human Parvovirus B19 and Rotavirus.

Virology Down Under - http://www.uq.edu.au/vdu/
An Australian based portal and educational site providing links and information on human viruses and related areas of science.

Hidden Killers--Deadly Viruses - http://library.thinkquest.org/23054/gather/index.shtml
Basics about viruses, our bodies' immune systems, information about specific viruses, and how these 'hidden killers' could potentially be used as bio-weapons. There are also weekly/bi-weekly feature articles on various facets that involve viruses.

A New Germ Theory - http://www.theatlantic.com/issues/99feb/germs.htm
The dictates of evolution virtually demand that the causes of some of humanity's chronic and most baffling "noninfectious" illnesses will turn out to be pathogens -- that is the radical view of a prominent evolutionary biologist.

EVA: European Virus Archive - http://www.european-virus-archive.com
Project aiming to create a European network of high calibre laboratories with the expertise to collect, amplify, characterise, standardise, authenticate, distribute and track all viruses.

Viper: Virus Particle Explorer - http://mmtsb.org/viper/
Descriptions of icosahedral virus capsid structures, along with structural and computational analysis.

VirHostNet - http://pbildb1.univ-lyon1.fr/virhostnet
Knowledgebase for virus-host and protein-protein interaction networks.

National Collection of Pathogenic Viruses - http://www.ukncc.co.uk/html/members/ncpv/ncpv.htm
NCPV preserves well-characterised, authenticated human pathogenic viruses in a secure facility. The agents or nucleic acids derived from these viruses are supplied to the worldwide scientific community according to national and international guidelines.

Baculovirus.com - http://www.baculovirus.com
Technical knowledge, information, newsgroup, helpful in using the Baculovirus expression vector system and insect cell expression.

Virology - http://www.elsevier.com/locate/issn/0042-6822
This journal publishes the results of basic research in all branches of virology, including the viruses of vertebrates and invertebrates, plants, bacteria, and yeasts/fungi.

What the Heck is a Virus? - http://people.ku.edu/~jbrown/virus.html
Provides a relatively easy to understand answer to this question.

Virusys Corporation - http://www.virusys.com
Specialists in the design and manufacture of virus related antibodies and antigens. A full range of laboratory services is available that includes infectious dose determination, viral protein purification and custom antibody production.

Blogging for Bacteriophages - http://www.phagehunter.org/
Blog by Tim Sampson who takes a look at recent developments in the world of microbiology.

The Molecules of HIV - http://www.mcld.co.uk/hiv/
The life cycle, immune response, and schematic diagram of the human immuno-deficiency virus.

Plant Virology - http://www.bioinvision.dk/plantvir1.html
Introduction to virus structure, transmission, and transport.

The Phage Phorum - http://www.phages.org
A place to find general information regarding biophages and an open discussion of topics related to biophages.

Veterinary Virology - http://www.pitt.edu/~super1/lecture/lec3351/001.htm
Lecture presentation on the replication of viruses within animals cells.

Viral Replication - http://www.epidemic.org/theFacts/viruses/viralReplication.html
Description of the hepatitis virus replication process.

Medical Virology - http://www.medicalvirology.com
Scientific findings, clinical therapies research, and public health aspects on viruses of medical importance.

GeneJohnson Inc. - http://www.genejohnson.net
HIV genomic data storage and analysis systems. HIVBase allows one to organize HIV sequence data, perform complex statistical analysis, and collaborate data all in one program.

Viralman - http://viralman.110mb.com
Educational site for students provided by H.Niazmand at Azad University of Kazeroun, with downloads and an image gallery.

Hepadnavirus Testing, Inc. - http://www.hvtinc.com

The site describes the services provided by Hepadnavirus Testing, Inc., which uses HBV and DHBV to evaluate disinfectants, inactivation processes, and antiviral drugs for the biotechnology and pharmaceutical industries.

Antiviral Intelistrat - http://www.antiviralintelistrat.com/
Database of antiviral drugs and vaccines. It regroups over 1700 product files containing detailed information on compounds and vaccines related to the prevention or treatment of viral diseases. Subscription needed.

Chicken Flu: Recombinant Genes on the Loose - http://www.synapses.co.uk/science/fluvirus.html
Information regarding viral genes, replication, and strain evolution. Written in 1997 but updated in 2005.

Seminars in Virology - http://www.sciencedirect.com/science?_ob=JournalURL&_cdi=7150&_auth=y&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=7ea28456e36b7099a712a2f126c0c294
Review journal dedicated to keeping scientists informed of developments in the field of virology on a topic by topic basis.

Virus Ultrastructure - http://web.uct.ac.za/depts/mmi/stannard/linda.html
This page is designed primarily as an introductory manual for students of virology,but anyone who is interested is invited to take a brief "peep" at some of the inhabitants of the microbial Lilliput Land.

Journal of General Virology - http://www.sgm.ac.uk/JGVDirect/
Archive of downloadable PDF virus journals.

Viral Protocols and Protocol Links (1)

Creation and Use of Infectious Virus Vector

http://www.stewartlab.net/Protocols/Making_Virus_1...

(Stewart Lab, Washington University at St. Louis)

Extraction of Geminiviral DNA from a Highly Mucilaginous Plant Protocol http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb18/R00-056.pdf

Protocol for the extraction of geminiviral DNA from a highly mucilaginous plant (Abelmoschus esculentus). Includes: Extraction; Alkali lysis; Cloning.

Generation of recombinant baculoviruses by co-transfection (Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)

http://www.its.caltech.edu/%7Ebjorker/Baculovirus-...
For co-transfection prepare at least 10mg of highly purified plasmid DNA. Spodoptera frugiperda cells are sensitive to some contaminants found in crude plasmid preparations, which cannot be removed by phenol/chloroform extraction or ethanol precipitation.

Gradient Purification of AT2 Inactivated HIV-1 Protocol http://ubik.microbiol.washington.edu/protocols/bl3/at2.htm

Protocol for gradient purification of AT2 inactivated HIV-1.

HIV-1 p24 Antigen Capture ELISA Protocol http://ubik.microbiol.washington.edu/protocols/bl3/p24elisa.htm

Protocol for HIV-1 p24 antigen capture ELISA.

Information Resources for Adjuvants and Antibody Production

http://www.nal.usda.gov/awic/pubs/antibody/
Article explaining the function of adjuvants, providing guidelines for their use, particularly Freund's adjuvants, and introducing some alternatives that are commercially available.

Immunohistochemistry: In Situ Hybridization

http://www.immunoportal.com/
Includes details of immunoenzyme techniques, immunofluorescence and in situ hybridizations, with a photograph gallery and discussion board.

Purification of Bacteriophage lambda Arms: Centrifugation through Sucrose Density Gradients Protocol

http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3990

Protocol is used to prepare the arms of any bacteriophage {lambda} vector.

Purification of Bacteriophage lambda Particles by Isopycnic Centrifugation through CsCl Gradients

http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3968

Isopycnic centrifugation through CsCl gradients is used to prepare infectious bacteriophage {lambda} particles of the highest purity that are essentially free of contaminating bacterial nucleic acids.

Purification of Recombinant Adeno-Associated Virus (rAAV) and Parovirus Protocol http://www.axis-shield.com/densityhome/optiprep/M04.pdf

Protocol for the purification of recombinant adeno-associated virus (rAAV) and parvovirus in pre-formed iodixanol gradients.

Retroviral infection of primary human keratinocytes (PHKs)

http://www.methodbook.net/cellcult/infectn.html

(Matt Lewis, Department of Pathology, University of Liverpool)

SDS-PAGE Sample Preparation of Adenovirus Infected Cells Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1752

Protocol for SDS-PAGE sample preparation of adenovirus infected cells.

TEV Purification Protocol http://openwetware.org/wiki/Keating:Experimental_Protocols:TEV_purification

Protocol for TEV purification.

Titration of Retrovirus Protocol

http://www.methodbook.net/virus/titratn.html

Protocol for the titration of retrovirus.

Viral Detection and Concentration in Food http://members.ift.org/NR/rdonlyres/69B323CE-B14C-4DD6-8F56-B25898C9EFB0/0/crfsfsv01n2p082085ms20010312.pdf

Methods and information related to viral detection and concentration in food. Includes: Nucleic acid amplification methods; Recent progress in polymerase chain reaction detection; Detection of human enteric viruses in non-shellfish food; Choice of primers for the detection of human caliciviruses in foods; Biosensors and microarray detection; Alternative confirmation methods.

Virion RNA Dot Blot Protocol

http://www.bio.com/protocolstools/protocol.jhtml?id=p617

Protocol describes the production of a dot blot membrane spotted with RNA isolated from virus particles.

Virus detection protocols for west nile virus in vertebrate and ...

www.ncbi.nlm.nih.gov/pubmed/12904372
Protocols to detect the presence of WNV have been refined since 1999 for ... Polymerase Chain Reaction/methods; RNA, Viral/isolation & purification ...

Virological Laboratory Techniques & Resources

(Source: All the Virology on the WWW)

BioGuide - PCR - general information about the polymerase chain reaction including primer design, hot-start, etc.

BioProtocol, Inc. - This site contains a large collection of high quality protocols for the life science researcher. These protocols include offerings from the virology field of study. The company has a highly reputable editorial board to oversee the quality of its protocol collection as well as an editorial staff committed to procuring and producing the highest quality protocols available on-line.

Detailed protocol for gold labeling proteins for electron microscopy. This protocol describes a modified antibody-protein A-gold technique that eliminates the aggregation problem that frequently occurs with the usual procedure.

Cell Biology Laboratory Manual by Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College, St. Peter, MN.

SPOTTER: a new tool to measure viral plaques. The plaque assay is a classical virological technique, originally developed for bacteriophages by Felix d'Herelle (1917). Dulbecco and Vogt subsequently adapted the assay for mammalian viruses, and it is still widely used for virus isolation and purification, and to determine viral titers. The plaque assay is also frequently used to study in vitro growth properties of mutant viruses. A freeware program,called SPOTTER, operating under Win95 and up, allows convenient and precise analysis of large numbers of plaques.

Protocol On-Line - an exhaustive collection of methods and protocols for molecular and cellular biology from academic and comercial laboratories. There is also a Q&A sections that may be helpful for solving lab technique problems.

Comprehensive Protocol Collection - This resource attempts to offer a comprehensive collection of molecular biology protocols. The site links to protocols either maintained directly by the Ambros Lab at Dartmouth or at other locations around the world.

The HHMI Virtual Lab: This interactive laboratory, developed with Shockwave, demonstrates how an enzyme-linked immunosorbent assay (ELISA) is carried out and some of the experimental problems that may be encountered. A free Shockwave plugin is required.

Molecular Biology Protocols - a General Resource from the NOAA of Molecular Methods, Manuals, and some great Links.

PCR in situ: New Technologies with Single Cell Resolution for the Detection and Investigation of Viral Latency and Persistance" from the University of Minnesota

PCR Jump Station - This site attempts to provide scientists with a comprehensive directory for the polymerase chain reaction (PCR) and related techniques.

Labexplorer.com is a new web site written by clinical laboratorians for clinical laboratorians. Designed to be a comprehensive resource for those interested in clinical laboratory practices, the site provides a mechanism of communication between laboratorians, access to experts in a variety of clinical fields, job and career information, and comprehensive links to laboratory sites, diagnostic companies, organizations & interesting topics.

Molecular Biology Protocols on the Web

a great collection of protocol links from the publishers of Molecular Biology Today.

Databases of Virology

(Source: Google directory)

The Journal of Virology Online - http://jvi.asm.org/
Online version of the Journal of Virology, the premier journal for peer-reviewed papers on virology research.

Retrovirology - http://www.retrovirology.com
A peer reviewed journal that publishes reviews and original research on retroviruses.

Virus Databases Online - http://www.ictvdb.rothamsted.ac.uk/
A comprehensive index of viruses provided by the International Committee on Taxonomy of Viruses. Also provides a picture gallery, identification tool and software tools.

All the Virology on the WWW - http://www.virology.net/
Links to virology information, collection of virus pictures, virology bookshop, jobs, and course notes.

UK Clinical Virology Network - http://www.clinical-virology.org
News, clinical tests, and current viruses in circulation.

HIV Sequence Database - http://www.hiv.lanl.gov/content/index
Collection HIV and related genetic sequence data and computer analyses - from the Los Alamos Natl. Lab.

American Society for Virology - http://www.asv.org/
A forum for investigators of human, animal, insect, plant, fungal and bacterial viruses in areas such as clinical, ecological, biological or biochemical research.

Search Results for DNA (2)

Grocott's Methenamine Silver (GMS) stain and troubleshooting

Sep 28, 2008 ... www.e-biotek.com ... Crosslinking Protocols · PCR Resource Online · Recombinant DNA · Protein Purification and Identification Protocols ...
www.e-biotek.com/.../178-grocotts-methenamine-silver-gms-stain-and-troubleshooting.html

FCM: CaspaTag Caspase Activity Protocol

Nov 26, 2008 ... www.e-biotek.com ... Crosslinking Protocols · PCR Resource Online · Recombinant DNA · Protein Purification and Identification Protocols ...
www.e-biotek.com/.../220-fcm-caspatag-caspase-activity-protocol.html

RNA FISH Protocols

Nov 30, 2008 ... www.e-biotek.com ... TRANSCRIPTION OF RNA · Electrophoresis Protocols (11-26) · DNA FISH Protocol · End Labeling Protocols (for DNA & RNA) ...
www.e-biotek.com/rna/228-rna-fish-protocols.html

Elegans Immunohistochemistry

Sep 28, 2008 ... www.e-biotek.com ... PCR Resource Online · Recombinant DNA · Protein Purification and Identification Protocols ...
www.e-biotek.com/.../177-elegans-immunohistochemistry.html

NIH Protocols for Immunohistochemistry

Dec 4, 2008 ... www.e-biotek.com ... PCR Resource Online · Recombinant DNA · Protein Purification and Identification Protocols ...
www.e-biotek.com/.../236-nih-protocols-for-immunohistochemistry.html

Protein Protein Crosslinking Protocols

Dec 12, 2008 ... www.e-biotek.com ... Online Tools · PCR Resource Online · Recombinant DNA · Protein Purification and Identification Protocols ...
www.e-biotek.com/.../238-protein-protein-crosslinking-protocols.html

Databases

DNA and Forensics. Monday, 12 January 2009 03:31 biology ... Any type of organism can be identified by examination of DNA sequences ... www.e-biotek.com ...
www.e-biotek.com/databases.html

Archive (1)

DNA Purification (glass milk vs electroelution). 8. DNA and RNA sequencing ... Isolation of DNA,RNA, and Protein simultaneously. ... www.e-biotek.com ...
www.e-biotek.com/databases/182-archive-1.html

Troubleshooting Guide for Immunohistochemistry

Oct 5, 2008 ... www.e-biotek.com ... DNA Isolation, Purification and Troubleshooting · Immunohistochemistry Stain for Frozen tissue ...
www.e-biotek.com/.../191-troubleshooting-guide-for-immunohistochemistry-.html

Membrane Potential Analysis by Flow Cytometry

Nov 26, 2008 ... www.e-biotek.com ... FCM ：Annexin V Protocol · FCM: Calcium Flux Analysis · FCM: CaspaTag Caspase Activity Protocol · DNA Analysis by Flow ...
www.e-biotek.com/.../224-membrane-potential-analysis-by-flow-cytometry.html

Troubleshooting Guide: Western Blotting

Oct 5, 2008 ... www.e-biotek.com ... DNA Isolation, Purification and Troubleshooting · Western Blot Protocol · Grocott's Methenamine Silver (GMS) stain and ...
www.e-biotek.com/.../190-troubleshooting-guide-western-blotting.html

TROUBLESHOOTING TIPS – Western Blotting - High background

Oct 5, 2008 ... www.e-biotek.com ... DNA Isolation, Purification and Troubleshooting · Western Blot Protocol · Grocott's Methenamine Silver (GMS) stain and ...
www.e-biotek.com/.../195-troubleshooting-tips--western-blotting.html?...

Inverse PCR

Sep 26, 2008 ... Cloning flanking DNA from pD991 enhancer trap T-DNA inserts ... E. Jay Rehm. Inverse PCR Trevor Epp ... www.e-biotek.com ...
www.e-biotek.com/pcr/71-inverse-pcr.html

PCR Methods

4, Handbook for DNA isolation, RAPD-PCR and PCR-RFLP, Administrator, 1249. 5, Introduction to PCR, Administrator, 853 ... www.e-biotek.com ...
www.e-biotek.com/pcr.html

Colony Hybridization Protocols

Aug 25, 2008 ... Here are some web links for Colony Hybridization Protocols.
www.e-biotek.com/dna/138-colony-hybridization-protocol.html

The Genetic Code

But, other DNA mutations can lead to errors in the protein that cause it to function abnormally and give rise to consequences as ... www.e-biotek.com ...
www.e-biotek.com/databases/243-the-genetic-code.html

H and E Stain Protocols

This is a collection of protocols for H and E stain. ... Home Page · DNA · RNA · PCR · Protein · CELL · Genetics · Immunology · Chromatography ...
www.e-biotek.com/.../173-h-and-e-stain-protocols.html

H & E Stain Troubleshooting

Home Page · DNA · RNA · PCR · Protein · CELL · Genetics · Immunology ... Home · Basic Technique H & E Stain Troubleshooting ... www.e-biotek.com ...
www.e-biotek.com/.../176-h-a-e-stain-troubleshooting.html

Tail Vein Injection Techniques

Sep 18, 2008 ... E-mail · Print · PDF. Page 1 of 3. Protocol 1. INTRAVENOUS TAIL VEIN INJECTION PROCEDURE FOR RATS AND MICE ... www.e-biotek.com ...
www.e-biotek.com/.../130-tail-vein-injection-techniques.html

Polysaccharide sequencing

E-mail · Print · PDF. Though polysaccharides are also biopolymers, it is not so common to talk of 'sequencing' a polysaccharide, ... www.e-biotek.com ...
www.e-biotek.com/.../121-polysaccharide-sequencing.html

Establishment and characterization of human embryonic stem cell line

E-mail · Print. Page 1 of 2. Blastocyst culture. Following in vitro fertilization (IVF) treatment, human pronuclear (PN) stage embryos ... www.e-biotek.com ...
www.e-biotek.com/.../257-establishment-and-characterization-of-human-embryonic-stem-cell-line-.html

Immunohistochemistry: Retrievals

Dec 15, 2008 ... www.e-biotek.com ... {Copyright (C) 2009 E-BioTek.Com. All Rights Reserved} · Contact Us · Sitemap · Google Sitemap.
www.e-biotek.com/.../241-immunohistochemistry-retrievals.html

H & E Stain Troubleshooting - Page 2

Home Page · DNA · RNA · PCR · Protein · CELL · Genetics · Immunology · Chromatography · Model Organism · Animal · Basic Technique ... E-mail · Print · PDF. Page 2 of 2. A. Hematoxylin is extracted from the wood of ... www.e-biotek.com ...
www.e-biotek.com/.../176-h-a-e-stain-troubleshooting.html?...

Chorioallantoic Membrane Vascular Assay

Sep 18, 2008 ... Chorioallantoic Membrane Vascular Assay. Thursday, 11 September 2008 09:46 biology. E-mail · Print · PDF. Page 1 of 2 ... www.e-biotek.com ...
www.e-biotek.com/.../148-chorioallantoic-membrane-vascular-assay.html

FACS for Bone marrow macrophages

E-mail · Print · PDF. 1. Remove cells from Valmark plates as usual. Wash x2 in FACS buffer: HBSS (no ... www.e-biotek.com ...
www.e-biotek.com/.../150-facs-for-bone-marrow-macrophages.html

B cell and T cell Isolation

Oct 12, 2008 ... www.e-biotek.com ... Variants of PCR (2) · Laboratory Manual for Biomedical Research · Handbook for DNA isolation, RAPD-PCR and PCR-RFLP ...
www.e-biotek.com/.../201-b-cell-and-t-cell-isolation-.html

BioProtocols--molecular biology

www.e-biotek.com ... DNA isolation & related protocols ... Isolation of DNA,RNA, and Protein simultaneously. DNA mutation detection by SSCP ...
www.e-biotek.com/protocols/ebiotek_313.htm

Radioimmunoassay--RIA Protocols

www.e-biotek.com ... Genomic DNA Isolation from Specific Samples · Mitochondrial DNA Isolation · Deoxyribose Isolation from DNA Degrasion · RNA Isolation ...
www.e-biotek.com/.../93-radioimmunoassay-ria-protocols.html

Arabidopsis Protocols

Arabidopsis genomic DNA prep-CTAB extraction method · Arabidopsis genomic DNA prep-Proteinase K method · Arabidopsis GUS staining ... www.e-biotek.com ...
www.e-biotek.com/model.../232-arabidopsis-protocols.html

All Mouse Models Federated Database

polymerase (DNA directed), delta 1, catalytic subunit [POLD1]. Point Mutant. targeted ... Submit search form. Web, www.e-biotek.com. Bo Blog Tags ...
www.e-biotek.com/.../233-all-mouse-models-federated-database.html

Biotechniques | Biotechniques

Nov 1, 2008 ... Whose DNA was analyzed to create the consortium's SNP map? Are SNP data available to the public? Tags: Genetics ... www.e-biotek.com ...
www.e-biotek.com/component/idoblog/viewtag/2.html

Protocols Online

Dec 21, 2009 ... Alternate method for purifying DNA from agarose gels ... Preparing proteins/antibodies/DNA/RNA for microinjection ... www.e-biotek.com ...
www.e-biotek.com/databases/254-protocols-online.html

Reaching the summit of protein dynamics - Biology Forum

Dec 15, 2009 ... DNA Methods ... RNA Methods ... PCR, RT-PCR, and Real Time PCR ... Genetics ... Microarray, Cellular Biology . ... www.e-biotek.com ...
www.e-biotek.com/bio-forum.html?func=view&catid=9&id...

BioProtocols--progress in life science

Progress in Life Science -- DNA in Cell. Contact Us: ebiotek.com@gmail.com {Copyright (C) 2008 E-BioTek.Com. All Rights Reserved} ...
www.e-biotek.com/protocols/ebiotek_232.htm

Newly identified enzymes help plants sense elevate - Biology Forum

Dec 15, 2009 ... DNA Methods ... RNA Methods ... PCR, RT-PCR, and Real Time PCR ... Protein Methods ... Genetics . ... www.e-biotek.com ...
www.e-biotek.com/bio-forum.html?func=view&id=60...36

Introduction to PCR

1 ul template DNA 5 ul 2 mM dNTP 8 ul 25 mM MgCl2 or MgSO4 (volume variable) water (to make up to 100 ul) ... www.e-biotek.com ...
www.e-biotek.com/pcr/91-introduction-to-pcr.html

Animal Search Engines

Sep 18, 2008 ... Tuesday, 08 July 2008 04:43 administrator. E-mail · Print · PDF · Animal Models & Strains Search Engine ... www.e-biotek.com ...
www.e-biotek.com/animal/94-animal-search-engines.html

Rep-PCR

Sep 26, 2008 ... E. coli Source Tracking by Rep-PCR DNA Fingerprinting The Regents of the University of Minnesota. How does Rep-PCR work ... www.e-biotek.com ...
www.e-biotek.com/pcr/48-rep-pcr.html

Influenza A(H1N1) virus - Biology Forum

DNA Methods ... RNA Methods ... PCR, RT-PCR, and Real Time PCR ... Protein Methods ... Genetics ... Microarray, Cellular Biology . ... www.e-biotek.com ...
www.e-biotek.com/bio-forum.html?func=view&id...

Electrophoresis Protocols (11-26)

It's a collection of protocols for DNA, RNA, and protein electrophoresis. ... www.e-biotek.com ... PCR analysis of DNA from Laser Microdissected Samples ...
www.e-biotek.com/basic.../171-electrophoresis-protocols-11-26.html

BioProtocols--basic lab techniques

Equipment V. Working with DNA VI. Sterile Technique VII. Working with E. coli. www.research.umbc.edu/~jwolf/method2.htm ... www.e-biotek.com ...
www.e-biotek.com/protocols/ebiotek_204.htm

Biomedical Blogs--Biologist's Own Space | Biotechniques

Whose DNA was analyzed to create the consortium's SNP map? Are SNP data available to the public? Tags: Genetics. Hits: 553 | Read more. ... www.e-biotek.com ...
www.e-biotek.com/bio-blog.html

Modified Immunohistochemical Protocol for Paraffin Embedded Sections

e. Cool slides for 30 minutes to 1 hour in the beaker. ... e. Check the level of buffer in the tray, fill up with buffer if necessary to ... www.e-biotek.com ... Immunohistochemistry: Retrievals · PCR Resource Online · Recombinant DNA ...
www.e-biotek.com/.../250-modified-immunohistochemical-protocol-for-paraffin-embedded-sections.html

BioProtocols--resource

Free Software pDRAW32 to Draw DNA Analysis Charts · Biological Educational Resources · Resources of Medical Biochemistry .... www.e-biotek.com ...
www.e-biotek.com/protocols/ebiotek_004.htm

Enzyme Assay（43-47） - Suding Lab Enzyme Assay Protocol

Sep 19, 2008 ... www.e-biotek.com ... Crosslinking Protocols · PCR Resource Online · Recombinant DNA · Protein Purification and Identification Protocols ...
www.e-biotek.com/.../162-enzyme-assay43-47.html?start...

Forum List - Biology Forum

Questions and Answers: Pertaining to H & E Stain ... DNA Methods ... RNA Methods ... PCR, RT-PCR, and Real Time PCR . ... www.e-biotek.com ...
www.e-biotek.com/bio-forum.html

BioProtocols--progress in gene therapy 1

www.e-biotek.com .... murine IL-10-expressing plasmid DNA in type-B gelatin nanoparticles, which were further encapsulated in poly(epsilon-caprolactone) ...
www.e-biotek.com/protocols/ebiotek_236.htm

Nanotechnology and Stem Cell Applications - Biology Forum

Genetic controls, using DNA or siRNA, is emerging as a useful tool for controlling cellular functions in stem cells, particularly for .... www.e-biotek.com ...
www.e-biotek.com/bio-forum.html?func=view&catid=15...

Reverse Transfection Protocols

Oct 16, 2008 ... www.e-biotek.com ... Cell Organelles and Cell Fractionation · DNA Methods （3） · Progress in RNA Interference Technology ...
www.e-biotek.com/.../202-reverse-transfection-protocols.html

Buffers and Solutions

3'-end Labeling of DNA with Klenow Fragment · 3'-end Labeling of Oligonucleotides with Terminal Transferase · 4% PARAFORMALDEHYDE ... www.e-biotek.com ...
www.e-biotek.com/buffer-and-solution.html

Electrophoresis Protocols (11-26) - Protocol for urea gel ...

It's a collection of protocols for DNA, RNA, and protein electrophoresis. ... www.e-biotek.com ... PCR analysis of DNA from Laser Microdissected Samples ...
www.e-biotek.com/.../171-electrophoresis-protocols-11-26.html?...

BioProtocols--biochemistry superbook

www.e-biotek.com ... DNA Replication, Recombination, and Repair (unit 1-64). The Immune System (unit 65-116). RNA Synthesis and Splicing (unit 117-166) ...
www.e-biotek.com/protocols/ebiotek_321.htm

THE ZEBRAFISH BOOK

E-mail · Print · PDF. A guide for the laboratory use of zebrafish Danio (Brachydanio) rerio by Monte Westerfield, Institute of ... www.e-biotek.com ...
www.e-biotek.com/ebooks/129-the-zebrafish-book.html

HPLC Troubleshooting

E-mail · Print · PDF. Troubleshooting High Performance Liquid Chromatography (from ASRG). 1. Peaks ... www.e-biotek.com ...
www.e-biotek.com/.../112-hplc-troubleshooting.html

Web Links of Protocols (1)

5'-end Labeling of DNA with T4 Polynucleotide Kinase ... A. Bst-catalyzed radiolabeled DNA sequencing ... www.e-biotek.com ...
www.e-biotek.com/databases/188-web-links-of-protocols-1.html

Databases of Biochemical Pathways

Dec 14, 2008 ... www.e-biotek.com ... {Copyright (C) 2009 E-BioTek.Com. All Rights Reserved} · Contact Us · Sitemap · Google Sitemap.
www.e-biotek.com/.../239-databases-of-biochemical-pathways.html

Cellular Biology

6, DNA Analysis by Flow Cytometry, Administrator, 606. 7, FCM: CaspaTag Caspase Activity Protocol, Administrator, 583 ... www.e-biotek.com ...
www.e-biotek.com/cell.html

Re:Influenza A(H1N1) virus - Biology Forum

DNA Methods ... RNA Methods ... PCR, RT-PCR, and Real Time PCR ... Protein Methods ... Genetics ... Microarray, Cellular Biology . ... www.e-biotek.com ...
www.e-biotek.com/bio-forum.html?func=view&id=54...34

Re:First Human Embryonic Stem Cell Lines Approved for - Biology Forum

DNA Methods ... RNA Methods ... PCR, RT-PCR, and Real Time PCR ... Protein Methods ... Genetics ... Microarray, Cellular Biology . ... www.e-biotek.com ...
www.e-biotek.com/bio-forum.html?func=view&catid=15...

First Human Embryonic Stem Cell Lines Approved for - Biology Forum

DNA Methods ... RNA Methods ... PCR, RT-PCR, and Real Time PCR ... Protein Methods ... Genetics ... Microarray, Cellular Biology . ... www.e-biotek.com ...
www.e-biotek.com/bio-forum.html?func=view&catid=15...

Directory (3)

FCM: DNA Analysis by Flow Cytometry. 17.Flow Cytometry：CaspaTag Caspase Activity ... Genetic Analyses for DNA Protein Interactions ... www.e-biotek.com ...
www.e-biotek.com/databases/183-directory-3.html

BioProtocols--progress in cancer apoptosis 1

www.e-biotek.com ... whereas 10 muM arsenite showed no recognizable effect on this nuclear transcription factor in the protein/DNA array analysis. ...
www.e-biotek.com/protocols/ebiotek_233.htm

Long PCR Protocol - Biology Forum

... /p/i/n/pinzheng/html/e-biotek/libraries/joomla/application/pathway.php on line 209 ...... It is a thermostable DNA polymerase, which possesses 3' to 5' ...
www.e-biotek.com/bio-forum.html?func=view&catid=8&id...

[PDF]

Western blot analysis using the Odyssey Infrared Imaging System

文件格式: PDF/Adobe Acrobat
Jan 11, 2009 ... small aliquot for DNA contamination detection. 5. Rinse the nuclear pellet with the lysis buffer once, then re-suspend in the lysis buffer ...
www.e-biotek.com/.../196-western-blot-analysis-using-the-odyssey-infrared-imaging-system-.pdf

Sitemap

Home Page · DNA · RNA · PCR · Protein · CELL · Genetics · Immunology · Chromatography · Model Organism · Animal · Basic Technique ... www.e-biotek.com ...
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