Biochemical Protocol.16. Arabidopsis Protocols

Arabidopsis cell suspension and root liquid culture

Arabidopsis EMS mutagenesis of seeds

Arabidopsis genomic DNA prep-CTAB extraction method

Arabidopsis genomic DNA prep-Proteinase K method

Arabidopsis GUS staining

Arabidopsis liquid culture

Arabidopsis mutants

Arabidopsis quick genomic DNA prep for PCR

Arabidopsis root culture

Arabidopsis seed sterilization

Arabidopsis vacuum infiltration (plant transformation)

Confocal Microscopy- Nuclear Staining

Cross-fertilization of Arabidopsis

Crossing Arabidopsis plants (flower emmansculation and flower preparation for fertilization)

Glucocorticoid (GVG)-inducible promoter system for Arabidopsis

How we grow Arabidopsis

In situ hybridization using radioactive probes

Non radioactive in situ hybridization

Plastic Sectioning- Tissue fixation

Protoplast preparation (plant tissue)

Scanning Electron Microscope- Tissue fixation and dissection

Transmission Electron Microscopy- Tissue fixation

Vacuum infiltration of Arabidopsis plants

Biochemical Protocol.17. Yeast Protocols (1)

96-Well Format Yeast Protein Purification Protocol

A microarray-based protocol for monitoring the growth of yeast .

A protocol for isolation and visualization of yeast nuclei by scanning electron microscopy (SEM).

BLAST Searching from SGD.

Brent Lab Two-Hybrid Information

Chromatin immunoprecipitation protocol for Saccharomyces cerevisiae.

Codon Usage Tables.

Colony PCR

Common Access to Biological Resources and Information (CABRI)

Commonly used lab strains

Competent Yeast Preparation

Confirmation PCR

Direct PCR from Whole Yeast Cells

DNA Replication Protocols from the Fangman and Brewer Labs, University of Washington.

DNA Replication Techniques

European announcement of the complete Sequence, April 24, 1996.

FASTA
S. cerevisiae protein structural information
Yeast Maps
Genomic View
Worm-Yeast Protein Comparison
Mammalian Homology to Yeast
Yeast Genome Restriction Analysis
Search Saccharomyces GenBank Sequences
Pairwise Chromosome Similarity View

FASTA Searching from SGD.

Gene Disruption via PCR
Confirmation PCR

GeneQuiz - Consortium for large scale sequence analysis.

Gietz Lab's Yeast Transformation Page

Growth Chamber for Time Lapse Imaging of Budding Yeast

GST Fusion Protein Purification from Yeast

Interaction Trap at Work

INTESTINAL DYSBIOSIS / YEAST PROTOCOL

List of species specific documents from SWISS-PROT.

National Collection of Yeast Cultures (NCYC)

NCRR Yeast Resource Center

Paul's Yeast CHIP

PCR to check genotype

Plasmid Rescue from Yeast from Liquid Culture and Clony Rescue

Preparation of Yeast Cell for Immunoeletron Microscopy

Protein Prep

Protein Search from MIPS.

Protocol for Yeast-Based Androgen Receptor Assay ...

Quick Total Yeast Protein Isolation for SDS-PAGE Gels

Biochemical Protocol.17. Yeast Protocols (2)

Saccharomyces Gene Registry at SGD.

Slide Stripping Protocol Agilent Yeast Arrays

Staining of yeast by Rhodamine-Phalloidin/Calcofluor

Sucrose Density Gradient Fractionation of Yeast Membranes

Systematic Sequencing Table f

The Definitive Yeast Transformation Homepage from the University of Manitoba.

The MGuide. Version 2.0

The World-Wide Web Virtual Library: Yeast

Tritech Research

VectorDB, collected sequences of cloning vectors for use in a variety of organisms.

Vectorette PCR of Yeast DNA

Whole Cell Extracts

Yale Genome Analysis Center

Yeast Actin Capture Assay

Yeast Actin Purification protocol

Yeast Cell Cycle Flow Cytometry Protocol

Yeast Cell Micromanipulation, a protocol for making a support rod with fiber optic needle attached.

Yeast Colony PCR

Yeast Genome Directory

Yeast Genome Project Information from MIPS.

Yeast GST-fusion Collection

Yeast Histone Prep (from Jakob Waterborg; .pdf format)

Yeast IF without Dehydration

Yeast Immunofluorescence protocol

Yeast Immunofluorescence with Methanol/Acetone Dehydration

Yeast Immunoprecipitation

Yeast Knockout (YKO) Collection

Yeast Lab Protocols from the Botstein Lab, Stanford University.

Yeast Lab Protocols from the Breeden Lab, Fred Hutchinson Cancer Research Center.

Yeast Lab Protocols from the Gottschling Lab, Fred Hutchinson Cancer Research Center.

Yeast Mitochondrial Protein Database

Yeast Nuclear Extract Prep (small scale)

Yeast Protein Preparation for SDS PAGE and Western (rapid)

Yeast Proteome Database (YPD), accessible only to subscribers.

Yeast ProtoArrayTM Proteome Chips

Yeast Protocols

Yeast Protocols from the Gottschling Lab

Yeast Protocols Handbook

Yeast Sequencing at the Sanger Centre.

Yeast Sporulation

Yeast Tet-promoters Hughes Collection

Yeast Transposon Insertion Library Collection

Yeast Two-Hybrid Analisys

Yeast Whole Cell Extract (small scale)

Yeast-GFP Clone Collection

Yeast-TAP Fusion Library.

Biochemical Protocol.18. Mice

129 Strain Nomenclature

Cre transgenic database

Jax mice in development

Knockout mouse database

Ly5 (CD45) mice
Complete Blood Cell Counts from Mice
Genotyping p27 knockout mice
Tail DNA prep
Toe ID System
Using the MPD
Necropsy Form
GE Micro CT scans

Mouse Pathology Database

NCI mouse models

p27 Knockout Mouse Info at Jax

Biochemical Protocol.19. Buffers and Solutions

SOLUTIONS 1.

Solutions 2.

Common Buffers*

Lab Recipes

BioChapter.20. Online Resources and Reference Databases (1)

List A (From: FEROLab)

Research Journals

Journal Search via Arnold Llibrary

Entrez-PubMed at NCBI

OVID

Community of Science Database

UW Library Catalog

Nobel Foundation

FHCRC Stockroom (FHCRC only)

FHCRC Genomics software

Methods & Reagents BIOSCI newsgroup

Technical Tips at BioMedNet

Antibody Resource Page

Science Advisory Board

Isoelectric Point Calculator

Primer3

Primer3+ Nucleic Acid Databases

Genbank

Eukaryotic Promoter Database

Mouse Genome

Human-Mouse Homology

David Gene Ontology

Panther

ExPASy

Protein Data Bank at Brookhaven

Histone Database

PROSITE

UCL DbBrowser

PhosphoBase

ProDom

OGlycBase

BioMagResBank Pairwise Alignment Servers

BLAST

UCSC Blat

BLAST 2 Sequences against each other

BLOCKS at FHCRC

Michigan Tech

The Pasteur Institute

ClustalW at EMBnet

BOXSHADE for easy viewing

The Pasteur Institute

Michigan Tech (Map)

UC Santa Cruz (SAM)

PDQ Clinical trials

FHCRC Clinical System

UW Mindscape

UWCores

UW Orca

Normal Lab Values

UW HealthLinks

R-project

BioConductor

Venn Diagram applet

Poisson Probability Calculator

BioChapter.20. Online Resources and Reference Databases (2)

List B. (From Biotechniques)

Free eBooks in life science

Method Books

Free eBooks in life science (1-4)

How to make buffers and solutions?

Most Updated Biomedical Books

Pathway databases

Animal search system

Pathway Search

Genetics Education and Handbook

Progress in Life Science

Biomedical Job Opportunities

Tools for Statistics

Free Software pDRAW32 to Draw DNA Analysis Charts

Biological Educational Resources

Resources of Medical Biochemistry

Textbooks and Lab Manuals

Cinema Online, Free Movies in Life Science --- (1) (2) (3)

Hot Links:
HHS.gov

PubMed
PLOS

Wikipedia
Glossary of Genetics

Cell
Science
Nature
Primer 3
SMS2
ImageJ
WHO
NIH
FDA
ATCC

IMAGE

NSCB

UniProt