Biochemical Protocol.17. Yeast Protocols (2)

Saccharomyces Gene Registry at SGD.

Slide Stripping Protocol Agilent Yeast Arrays

Staining of yeast by Rhodamine-Phalloidin/Calcofluor

Sucrose Density Gradient Fractionation of Yeast Membranes

Systematic Sequencing Table f

The Definitive Yeast Transformation Homepage from the University of Manitoba.

The MGuide. Version 2.0

The World-Wide Web Virtual Library: Yeast

Tritech Research

VectorDB, collected sequences of cloning vectors for use in a variety of organisms.

Vectorette PCR of Yeast DNA

Whole Cell Extracts

Yale Genome Analysis Center

Yeast Actin Capture Assay

Yeast Actin Purification protocol

Yeast Cell Cycle Flow Cytometry Protocol

Yeast Cell Micromanipulation, a protocol for making a support rod with fiber optic needle attached.

Yeast Colony PCR

Yeast Genome Directory

Yeast Genome Project Information from MIPS.

Yeast GST-fusion Collection

Yeast Histone Prep (from Jakob Waterborg; .pdf format)

Yeast IF without Dehydration

Yeast Immunofluorescence protocol

Yeast Immunofluorescence with Methanol/Acetone Dehydration

Yeast Immunoprecipitation

Yeast Knockout (YKO) Collection

Yeast Lab Protocols from the Botstein Lab, Stanford University.

Yeast Lab Protocols from the Breeden Lab, Fred Hutchinson Cancer Research Center.

Yeast Lab Protocols from the Gottschling Lab, Fred Hutchinson Cancer Research Center.

Yeast Mitochondrial Protein Database

Yeast Nuclear Extract Prep (small scale)

Yeast Protein Preparation for SDS PAGE and Western (rapid)

Yeast Proteome Database (YPD), accessible only to subscribers.

Yeast ProtoArrayTM Proteome Chips

Yeast Protocols

Yeast Protocols from the Gottschling Lab

Yeast Protocols Handbook

Yeast Sequencing at the Sanger Centre.

Yeast Sporulation

Yeast Tet-promoters Hughes Collection

Yeast Transposon Insertion Library Collection

Yeast Two-Hybrid Analisys

Yeast Whole Cell Extract (small scale)

Yeast-GFP Clone Collection

Yeast-TAP Fusion Library.

Biochemical Protocol.18. Mice

129 Strain Nomenclature

Cre transgenic database

Jax mice in development

Knockout mouse database

Ly5 (CD45) mice
Complete Blood Cell Counts from Mice
Genotyping p27 knockout mice
Tail DNA prep
Toe ID System
Using the MPD
Necropsy Form
GE Micro CT scans

Mouse Pathology Database

NCI mouse models

p27 Knockout Mouse Info at Jax

Biochemical Protocol.19. Buffers and Solutions

SOLUTIONS 1.

Solutions 2.

Common Buffers*

Lab Recipes

BioChapter.20. Online Resources and Reference Databases (1)

List A (From: FEROLab)

Research Journals

Journal Search via Arnold Llibrary

Entrez-PubMed at NCBI

OVID

Community of Science Database

UW Library Catalog

Nobel Foundation

FHCRC Stockroom (FHCRC only)

FHCRC Genomics software

Methods & Reagents BIOSCI newsgroup

Technical Tips at BioMedNet

Antibody Resource Page

Science Advisory Board

Isoelectric Point Calculator

Primer3

Primer3+ Nucleic Acid Databases

Genbank

Eukaryotic Promoter Database

Mouse Genome

Human-Mouse Homology

David Gene Ontology

Panther

ExPASy

Protein Data Bank at Brookhaven

Histone Database

PROSITE

UCL DbBrowser

PhosphoBase

ProDom

OGlycBase

BioMagResBank Pairwise Alignment Servers

BLAST

UCSC Blat

BLAST 2 Sequences against each other

BLOCKS at FHCRC

Michigan Tech

The Pasteur Institute

ClustalW at EMBnet

BOXSHADE for easy viewing

The Pasteur Institute

Michigan Tech (Map)

UC Santa Cruz (SAM)

PDQ Clinical trials

FHCRC Clinical System

UW Mindscape

UWCores

UW Orca

Normal Lab Values

UW HealthLinks

R-project

BioConductor

Venn Diagram applet

Poisson Probability Calculator

BioChapter.20. Online Resources and Reference Databases (2)

List B. (From Biotechniques)

Free eBooks in life science

Method Books

Free eBooks in life science (1-4)

How to make buffers and solutions?

Most Updated Biomedical Books

Pathway databases

Animal search system

Pathway Search

Genetics Education and Handbook

Progress in Life Science

Biomedical Job Opportunities

Tools for Statistics

Free Software pDRAW32 to Draw DNA Analysis Charts

Biological Educational Resources

Resources of Medical Biochemistry

Textbooks and Lab Manuals

Cinema Online, Free Movies in Life Science --- (1) (2) (3)

Hot Links:
HHS.gov

PubMed
PLOS

Wikipedia
Glossary of Genetics

Cell
Science
Nature
Primer 3
SMS2
ImageJ
WHO
NIH
FDA
ATCC

IMAGE

NSCB

UniProt

Web Source of Biochemical Protocols

Web links of bioprotocols (1)

Web links of bioprotocols (2)

Protocols for Protein
1 Enzyme Assay（43-47）
2 Enzyme Assay（36-42）
3 Enzyme Assay（22-28）
4 Protocols for In Vitro Translation
5 Dot Blot Protocols
6 Selected Protocols of Protein Purification
7 Western Blotting Protocols (1)
Protocols for Cells
1 FACS for Bone marrow macrophages
2 Bone Marrow Macrophages
3 Metabolic Labeling of Cells with 35S
4 Protocols from the science advisory board
5 Introduction to Animal Cell Culture
6 Cell Transfection Protocols
7 Laser Capture Microdissection Protocols
8 Stem Cell Research Techniques and Protocols
9 XTT Cell Viability Assay Protocol
10 Apoptosis Assay Protocols
Protocols for Genetics
1 Genetic Analyses for DNA Protein Interactions
2 Comet Assay
3 Epigenetics Protocols
Protocols for Immunology and IHC
1 Grocott's Methenamine Silver (GMS) stain and troubleshooting
2 Elegans Immunohistochemistry
3 Cytokine Assay Protocols
4 Fluorescence in situ hybridization (FISH)
5 Radioimmunoassay--RIA Protocols
6 Competitive ELISA Protocols
7 Immunohistochemistry Stain for Frozen tissue
Protocols for Chromatography
1 Polysaccharide sequencing
2 Application Guides and Protocols (Nest Group)
3 HPLC Troubleshooting
4 Introduction to HPLC
Protocols for Model Organisms
1 Yeast Protocols
Protocols for Animal Experiment
1 Murine Models of Human Breast Cancer (B)
2 Chorioallantoic Membrane Vascular Assay
3 Guidelines for Techniques with Rodents (Duke University)
4 Tail Vein Injection Techniques
5 Animal Search Engines
Protocols for Basic Techniques
1 H & E Stain Troubleshooting
2 H and E Stain Protocols
3 Electrophoresis Protocols (11-26)
4 Lab. Safety in Life Science

Biorotocols for Biochemistry

Protocols for DNA
1 Methylene Blue DNA staining protocol
2 Plasmid Protocols--(31-37)
3 Plasmid Protocols--(21-30)
4 Plasmid Protocol--(1-10)
5 Microsatellites Protocols
6 In-Situ hybridization to Embryonic Tissue Section
7 DNA ligation Protocol
8 Colony Hybridization Protocols
9 Colony Hybridization
10 DNA Isolation, Purification and Troubleshooting
11 Deoxyribose Isolation from DNA Degrasion
12 Mitochondrial DNA Isolation
13 Genomic DNA Isolation from Specific Samples
14 Genome DNA Extraction
Protocols for RNA
1 IN VITRO TRANSCRIPTION OF RNA
2 SINGLE CELL mRNA AMPLIFICATION (REVERSE NORTHERN ANALYSIS)
3 Protocols for SiRNA
4 SELEX—in vitro selection
5 Nucleic Acids Research Methods (2)
6 RNA MicroArray Protocol
7 RNA Isolation
Protocols for PCR
1 PCR Based Molecular Cloning
2 Handbook for DNA isolation, RAPD-PCR and PCR-RFLP
3 Introduction to PCR
4 General PCR introduction
5 PCR Animations
6 PCR, RT PCR and Real Time PCR Tutorials
7 PCR Application Manual
8 PCR Elisa
9 PCR RFLP
10 PCR SSCP
11 Real Time PCR
12 RT-PCR Protocols
13 Variants of PCR (2)
14 Variants of PCR (1)
15 AFLP PCR
16 Alu-PCR
17 Asymmetric PCR
18 Colony PCR Protocols
19 Competitive and Quantitative RT-PCR
20 Degenerate PCR
21 Differential Display PCR
22 In Situ PCR
23 Inverse PCR
24 Ligation Mediated Suppression PCR
25 Long PCR Protocols
26 Methylation Specific PCR
27 Multiplex PCR
28 Nested PCR
29 RACE PCR
30 RAPD PCR
31 Rep-PCR
32 TAIL PCR
33 Touchdown PCR
34 Vectorette PCR